{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T16:09:58Z","timestamp":1771430998958,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T00:00:00Z","timestamp":1736380800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      People with cystic fibrosis (CF) may not expectorate sputum at young ages or after they receive CFTR modulators. While oropharyngeal swabs are commonly used to test for lower airway pathogens, it is unknown whether\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      from the oropharynx matches the strain(s) infecting the lungs. Our goal was to determine whether oropharyngeal and sputum isolates of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      are genetically distinct in a cohort of patients with CF.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We obtained historical\n                      <jats:italic>S. aureus<\/jats:italic>\n                      isolates from patients who intermittently expectorated sputum in 2018, and we prospectively cultured\n                      <jats:italic>S. aureus<\/jats:italic>\n                      from oropharyngeal swabs and sputum from subjects with CF between August 2020 and February 2022. We performed short\u2010read whole genome sequencing, determined sequence type, and performed phylogenetic analysis using\n                      <jats:italic>S. aureus<\/jats:italic>\n                      core genome single nucleotide polymorphisms (SNPs). We assigned isolates from a patient to the same strain if they had the same sequence type and differed by \u2264\u200960 SNPs or the isolates were not disturbed by clade breaker analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      36 subjects had\n                      <jats:italic>S. aureus<\/jats:italic>\n                      in \u2265\u20091 oropharyngeal swab and \u2265\u20091 sputum in 2018. In the prospective collection, 31 subjects had synchronous oropharyngeal swab and sputum collections. Although polyclonal infections were detected, sputum and oropharyngeal isolates of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      typically matched the same strain within study subjects, both over the span of 2018 (31\/36 patients) and when collected simultaneously from 2020 to 2022 (29\/31 patients).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In patients with CF who intermittently produce sputum, oropharyngeal swabs identify\n                      <jats:italic>S. aureus<\/jats:italic>\n                      with genetic and phenotypic similarity to those cultured from sputum.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ppul.27475","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T07:28:15Z","timestamp":1736407695000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Concordance of\n                    <i>Staphylococcus aureus<\/i>\n                    From Oropharyngeal and Sputum Cultures in People With Cystic Fibrosis"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4015-9695","authenticated-orcid":false,"given":"Christian F.","family":"Zirbes","sequence":"first","affiliation":[{"name":"Stead Family Department of Pediatrics University of Iowa Carver College of Medicine Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andries","family":"Feder","sequence":"additional","affiliation":[{"name":"Children's Hospital of Philadelphia Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony J.","family":"Pamatmat","sequence":"additional","affiliation":[{"name":"Stead Family Department of Pediatrics University of Iowa Carver College of Medicine Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa R.","family":"Bartels","sequence":"additional","affiliation":[{"name":"Stead Family Department of Pediatrics University of Iowa Carver College of Medicine Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Pitcher","sequence":"additional","affiliation":[{"name":"Stead Family Department of Pediatrics University of Iowa Carver College of Medicine Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis L.","family":"Rozen","sequence":"additional","affiliation":[{"name":"Stead Family Department of Pediatrics University of Iowa Carver College of Medicine Iowa City Iowa 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Iowa Carver College of Medicine Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jared J.","family":"Hill","sequence":"additional","affiliation":[{"name":"Stead Family Department of Pediatrics University of Iowa Carver College of Medicine Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lindsey D.","family":"Reinhardt","sequence":"additional","affiliation":[{"name":"Stead Family Department of Pediatrics University of Iowa Carver College of Medicine Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos L.","family":"Oberto","sequence":"additional","affiliation":[{"name":"Stead Family Department of Pediatrics University of Iowa Carver College of Medicine Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Boyken","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Iowa Carver College of Medicine Iowa 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