{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T16:09:20Z","timestamp":1771430960970,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Cellular characteristics of induced sputum (IS) are not investigated in cystic fibrosis (CF) patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This pilot study, conducted on 17 expectorating CF adolescents, compared sputa obtained the same day, in a stable period, by autogenic drainage (expectorating sputum, ES) and 4\u2009h later after inhaling hypertonic saline (IS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      No difference was noted concerning weight, volume, and percentage of dead cells between the two collection methods. Sample quality (&lt;\u200950% of dead cells and &lt;\u200920% squamous cells) was higher in the case of IS than ES (94.1% vs. 58.8%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.03), with a doubled cell count (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01), a higher proportion of alveolar macrophages (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.03), and a lower proportion of squamous cells (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.004). The detection of germs increased by 44% in IS samples, possibly modifying therapeutic management in 17.6% of the patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>IS improves the quality and the microbiological detection of the sample, even among CF patients who spontaneously expectorate.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ppul.27488","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T08:26:55Z","timestamp":1737448015000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Induced Sputum: A Valuable Tool for Assessing Cellular and Microbiological Characteristics in Cystic Fibrosis Expectorating Teenagers"],"prefix":"10.1002","volume":"60","author":[{"given":"M.","family":"Guedes","sequence":"first","affiliation":[{"name":"Centre de Ressources et de Comp\u00e9tences pour la Mucoviscidose, CHU Timone\u2010Enfants Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Frankel","sequence":"additional","affiliation":[{"name":"Service de Biologie Cellulaire APHM, INSERM, MMG, H\u00f4pital la Timone Aix Marseille Universit\u00e9 Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. A.","family":"Baron","sequence":"additional","affiliation":[{"name":"IHU M\u00e9diterran\u00e9e Infection, IRD, APHM, MEPHI, Facult\u00e9 de M\u00e9decine et de Pharmacie Aix Marseille Univ Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Berbis","sequence":"additional","affiliation":[{"name":"UR 3279 CEReSS, Support Unit for Clinical Research and Economic Evaluation, APHM Aix\u2010Marseille Univ Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Stremler\u2010Le Bel","sequence":"additional","affiliation":[{"name":"Centre de Ressources et de Comp\u00e9tences pour la Mucoviscidose, CHU Timone\u2010Enfants Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Baravalle\u2010Einaudi","sequence":"additional","affiliation":[{"name":"Centre de Ressources et de Comp\u00e9tences pour la Mucoviscidose, CHU Timone\u2010Enfants Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Roll","sequence":"additional","affiliation":[{"name":"Service de Biologie Cellulaire APHM, INSERM, MMG, H\u00f4pital la Timone Aix Marseille Universit\u00e9 Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Kaspi","sequence":"additional","affiliation":[{"name":"Service de Biologie Cellulaire APHM, INSERM, MMG, H\u00f4pital la Timone Aix Marseille Universit\u00e9 Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Dubus","sequence":"additional","affiliation":[{"name":"Centre de Ressources et de Comp\u00e9tences pour la Mucoviscidose, CHU Timone\u2010Enfants Marseille France"},{"name":"C2VN, INSERM 1263, INRAE 1260 Aix\u2010Marseille Universit\u00e9 Mraseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2013.05.013"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(18)30171-1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2022.01.006"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.10226"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2010.06.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23150"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.07-2259"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201203-0507OC"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.26023"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000111818"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.3.9908057"},{"key":"e_1_2_9_13_1","unstructured":"European Cystic Fibrosis Society Clinical Trial Network (ECFS\u2010CTN\/2.4\/001). Sputum Induction. Standard Operating Procedure. 2014: 1\u201322."}],"container-title":["Pediatric Pulmonology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ppul.27488","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ppul.27488","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ppul.27488","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T15:28:11Z","timestamp":1771428491000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ppul.27488"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/ppul.27488"],"URL":"https:\/\/doi.org\/10.1002\/ppul.27488","archive":["Portico"],"relation":{},"ISSN":["8755-6863","1099-0496"],"issn-type":[{"value":"8755-6863","type":"print"},{"value":"1099-0496","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-06-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e27488"}}