{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T05:19:45Z","timestamp":1788239985835,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["1 PO1 HL128192"],"award-info":[{"award-number":["1 PO1 HL128192"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["1 PO1 HL158507"],"award-info":[{"award-number":["1 PO1 HL158507"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Decreased apical bicarbonate transport into the airway surface liquid (ASL) has been associated with decreased ASL pH, which can have adverse respiratory effects. However, the human CF epithelium can normalize ASL pH. Thus, we hypothesized that pH regulatory proteins other than the CFTR could be altered in the CF epithelium.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Primary human nasal and bronchial epithelial cells from healthy controls and CF subjects were grown at air\u2010liquid interface until fully ciliated. Western blot was used to measure proteins known to affect human epithelial pH: carbonic anhydrases (CA) 1, 2, and 12; voltage\u2010gated proton channel (Hv1); lactate dehydrogenases (LDH) A, B, D; dual oxidases (DUOX) 1, 2; Na\n                      <jats:sup>+<\/jats:sup>\n                      \/H\n                      <jats:sup>+<\/jats:sup>\n                      exchange regulatory factor; potassium\u2010transporting ATPase alpha chain 2 (ATP12A), S\u2010nitrosoglutathione reductase (GSNOR); glutaminase; vacuolar\u2010type ATPase (VTP\u2010ase); and NADPH oxidases (NOX) 1\u20104.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      NOX2 was decreased in CF\n                      <jats:italic>nasal<\/jats:italic>\n                      epithelial cells compared to controls. CA1, CA2, CA12, Hv1, LDHA, LDHD, ATP12A, GSNOR and NOX4 expression were increased in CF\n                      <jats:italic>bronchial<\/jats:italic>\n                      epithelial cells relative to the controls. Beta actin expression was variable, making normalization challenging.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Expression of CA's, Hv1, LDH's, ATP12A, GSNOR, and NOX4 were generally increased in bronchial epithelium from patients with CF. These proteins could serve to normalize the CF epithelial pH. Nasal cells did not have these changes, arguing that findings from primary nasal cells in culture cannot uniformly be extrapolated to understand the biology of primary bronchial epithelial cells in culture.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ppul.71784","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T08:05:47Z","timestamp":1786608347000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Human Nasal and Bronchial Epithelium in Cystic Fibrosis: Differences in Expression of Airway pH Regulatory Proteins"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9387-1961","authenticated-orcid":false,"given":"Michael D.","family":"Davis","sequence":"first","affiliation":[{"name":"Department of Pediatric Pulmonology, Allergy, and Sleep Medicine, Riley Hospital for Children Indiana University Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Allergy, and Sleep Medicine, Riley Hospital for Children Indiana University Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Allergy, and Sleep Medicine, Riley Hospital for Children Indiana University Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania Abdul","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Rainbow Babies and Children's Hospital Case Western Reserve University Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faiza","family":"Khalid","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care, Rainbow Babies and Children's Hospital Case Western Reserve University Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4495-4693","authenticated-orcid":false,"given":"James","family":"Chmiel","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Allergy, and Sleep Medicine, Riley Hospital for Children Indiana University Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Boyne","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Sleep Medicine Ann &amp; Robert H. 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