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After a 2\u2005h exposure, cells were still viable and 43% of spots (<jats:italic>n<\/jats:italic>\u2005=\u20051042) showed a significant change in protein level. We identified 59 spots whose expression had changed significantly, and these reflected cytoskeleton damage, mitochondrial lysis and endoplasmic reticulum stress. Actin filament assembly was disrupted as evidenced by an increase in both actin subunits and phosphorylated cofilin, whilst levels of tropomyosin, tropomodulin and actin related protein 2\/3 complex subunit decreased. Lower levels of ATP synthase subunits and mitochondrial inner membrane protein were identified as markers of mitochondrial lysis. Levels of various stress response proteins rose and, uniquely, levels of Ca<jats:sup>2+<\/jats:sup> binding proteins such as translationally controlled tumor protein rose and hippocalcin\u2010like protein 1 decreased. This response may have mitigated effects brought about by mitochondrial lysis and endoplasmic reticulum stress, and delayed or prevented apoptosis in NB\u20104 cells. These results resemble findings of similar proteomics studies in murine macrophages, although quantitative differences were observed.<\/jats:p>","DOI":"10.1002\/prca.200700074","type":"journal-article","created":{"date-parts":[[2007,9,11]],"date-time":"2007-09-11T12:48:55Z","timestamp":1189514935000},"page":"1266-1279","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Proteomic analysis of the response of the human neutrophil\u2010like cell line NB\u20104 after exposure to anthrax lethal toxin"],"prefix":"10.1002","volume":"1","author":[{"given":"Jun X.","family":"Wheeler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gail","family":"Whiting","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sjoerd","family":"Rijpkema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909093411107"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0706.010604"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.12.004"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5364.734"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20031382"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1128\/iai.47.1.306-310.1985","article-title":"Effects of anthrax toxin components on human neutrophils","volume":"47","author":"O'Brien J.","year":"1985","journal-title":"Infect. 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