{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:26:47Z","timestamp":1758637607570},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,9,11]],"date-time":"2007-09-11T00:00:00Z","timestamp":1189468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grieshaber-Foundation, Switzerland"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glaucomatous damage is a neurodegenerative eye disease and one of the leading causes of blindness with 67 million patients worldwide. Major currently challenging questions include early diagnosis, risk evaluation, and follow\u2010up. Circulating leukocytes have been demonstrated as potentially important source of disease specific markers. The relevance of expression alterations in leukocytes for glaucomatous damage needs to be clarified. Noteworthy, gene expression patterns of trabecular meshwork and Schlemm's canal, which are anatomically and functionally highly relevant for glaucoma pathology, were shown to be similar to those of circulating leukocytes. Here, we report extensive alterations in characteristic protein expression patterns of circulating leukocytes for Normal\u2010Tension and Primary Open\u2010Angle Glaucoma, as revealed by analysis of 2\u2010D PAGE images. Among most conservative alterations we found the protein spot identified by MALDI\u2010TOF as basic transcription factor activating protein\u20102beta (AP\u20102\u03b2). Western\u2010blot analysis demonstrated significantly increased protein expression rates of AP\u20102\u03b2 in both Normal\u2010Tension and Primary Open\u2010Angle Glaucoma <jats:italic>versus<\/jats:italic> controls. AP proteins are essential factors of the basic transcription regulation; AP\u20102 proteins play a decisive role, particularly, in morphogenesis of eye. Conservative AP\u20102 up\u2010regulation is of special importance in terms of basic transcriptional dysregulation that might be specific for glaucoma disease.<\/jats:p>","DOI":"10.1002\/prca.200700150","type":"journal-article","created":{"date-parts":[[2007,9,11]],"date-time":"2007-09-11T12:48:59Z","timestamp":1189514939000},"page":"1316-1323","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Disease proteomics reveals altered basic gene expression regulation in leukocytes of Normal\u2010Tension and Primary Open\u2010Angle glaucoma patients"],"prefix":"10.1002","volume":"1","author":[{"given":"Olga","family":"Golubnitschaja","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Yeghiazaryan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Wunderlich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans H.","family":"Schild","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Flammer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2005.081224"},{"key":"e_1_2_1_3_2","author":"Flammer J.","year":"2007","journal-title":"Surv. 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