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SW1990 and PANC\u20101 represent the two cancer cell lines, which are both derived from pancreatic duct, but at different cell differentiation stages. In this study, we applied the iTRAQ\u2010labeling technology and 2\u2010D strong cation exchange\/reversed phase liquid chromatography \u2013 LC\u2010MS\/MS) to profile the secreted proteins of SW1990 and PANC\u20101 cells in a conditioned cell culture medium. A total of 401 proteins were identified by MS\/MS and protein database searching, the percentages of these proteins predicted in the categories of plasma membrane, intracellular and secreted proteins were 29.2, 32.7 and 38.2%, respectively. Fifty six proteins were identified with unknown functions and 19 proteins were quantified with significant level changes between the two cancer cell lines under the specific cell condition with 12 proteins being up\u2010regulated (&gt;1.3\u2010fold change) in PANC\u20101 (<jats:italic>e.g.<\/jats:italic> FLJ31222 protein, 97\u2009kDa protein, type IV collagenase precursor, 38\u2009kDa protein and centaurin) and seven proteins being up\u2010regulated in SW1990 (<jats:italic>e.g.<\/jats:italic> fibroblast growth factor receptor substrate 2, putative p150, hypothetical protein LOC 654463 and LOC 55701). The proteins with significant level changes may provide a baseline to investigate mechanisms underlying the differentiation of two cell lines and can be further screened for better protein biomarkers in pancreatic cancer.<\/jats:p>","DOI":"10.1002\/prca.200800029","type":"journal-article","created":{"date-parts":[[2009,7,10]],"date-time":"2009-07-10T03:22:18Z","timestamp":1247196138000},"page":"862-871","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Profiling the potential biomarkers for cell differentiation of pancreatic cancer using iTRAQ and 2\u2010D LC\u2010MS\/MS"],"prefix":"10.1002","volume":"3","author":[{"given":"Haijing","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyan","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoqiang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peixuan","family":"Bi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhili","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,7,9]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.57.1.43"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(97)89547-5"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500178-MCP200"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr050375p"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.4.2.239"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-3000fje"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500091"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300814"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.19.5807-5814.2003"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/13690"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M400129-MCP200"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr060416x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600209"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr049821j"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M600398-MCP200"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01234.2006"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-4-38"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19981001)83:7<1376::AID-CNCR15>3.0.CO;2-1"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semradonc.2005.04.001"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006676-198810000-00003"},{"key":"e_1_2_7_22_2","first-page":"4393","article-title":"Establishment and characterization of human pancreatic adenocarcinoma cell line SW\u20101990 in tissue culture and the nude mouse","volume":"43","author":"Kyriazis A. 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