{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:28:22Z","timestamp":1762100902477},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,2,27]],"date-time":"2009-02-27T00:00:00Z","timestamp":1235692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human myometrium undergoes a major phenotypic change at labour likely involving modifications to key regulatory proteins. In some cases, the myometrium fails to activate normally and medical intervention is required to induce labour. In this study, 2\u2010D DIGE was used to examine changes in the myometrial proteome at the time of spontaneous (SL) and induced labour (IL). Proteomic profiles of nonlabouring term myometria (NL, <jats:italic>n<\/jats:italic>\u2005=\u20056) were quantitatively compared to SL (<jats:italic>n<\/jats:italic>\u2005=\u20056) and prostaglandin\/oxytocin\u2010IL term myometria (<jats:italic>n<\/jats:italic>\u2005=\u20056). In SL samples, 23 differentially expressed protein spots were detected (9 increased\/14 decreased compared to NL, <jats:italic>p<\/jats:italic>&lt;0.05). In IL samples, 59 differentially expressed spots were observed (13 increased\/46 decreased compared to NL). Comparison of SL and IL proteomes revealed 69 differentially expressed proteins (7 increased\/62 decreased). Two proteins consistently decreased in SL and IL samples were identified as transgelin (1.98\u2010 and 1.97\u2010fold decrease in SL and IL, respectively) and \u03b1B\u2010crystallin (3.27\u2010 and 2.49\u2010fold decrease). Levels of desmin and cytosolic phospholipase A2 \u03b2 were decreased 2.9\u2010 and 2.65\u2010fold, respectively only in IL samples. Our results show human labour is accompanied by general downregulation of specific myometrial proteins. Differences exist between SL and IL myometrial proteomes indicating divergence of underlying processes and highlighting the importance of distinguishing these groups in future studies of parturition. Our findings underscore the utility of discovery approaches in investigations of organ\u2010wide protein changes that underlie discrete physiological events including human labour.<\/jats:p>","DOI":"10.1002\/prca.200800050","type":"journal-article","created":{"date-parts":[[2009,2,27]],"date-time":"2009-02-27T12:17:14Z","timestamp":1235737034000},"page":"288-298","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Spontaneous and induced labour are associated with different myometrial proteomes in the human"],"prefix":"10.1002","volume":"3","author":[{"given":"David A.","family":"MacIntyre","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Yeo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Kwek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew M.","family":"Bisits","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eng\u2010Cheng","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,2,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1046\/j.1471-0528.2003.00023.x","article-title":"Mechanisms of labour\u2013biochemical aspects","volume":"110","author":"Lopez Bernal A.","year":"2003","journal-title":"BJOG"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsgi.2005.07.002"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/6.12.1141"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1210\/jcem.87.6.8439","article-title":"Human myometrial genes are differentially expressed in labor: A suppression subtractive hybridization study","volume":"87","author":"Chan E. 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