{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:52:43Z","timestamp":1789937563367,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,10,22]],"date-time":"2008-10-22T00:00:00Z","timestamp":1224633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neurocysticercosis (NC) invokes formidable neurological problems worldwide. Previous proteomic analyses revealed most of the low\u2010molecular\u2010weight proteins might derive from two macromolecules of 120\u2005kDa (consisting of 14\u201338\u2005kDa subunits) and 150\u2005kDa (7\u201315\u2005kDa subunits) of <jats:italic>Taenia solium<\/jats:italic> metacestode (TsM) cyst fluid (CF). We characterized serological properties of these two proteins and established an immunopotent chimera. The 120 and 150\u2005kDa proteins harbored 54\u201381 and 94\u201398% of the antibody\u2010binding activity of the crude CF with minimal antigenic cross\u2010reactivity to each other. The expression and immune recognition of the 150\u2005kDa subunits were relatively constant, regardless of the different geographical origins of the CF collected, while those of the 120\u2005kDa subunits varied by their origins (Asia <jats:italic>vs.<\/jats:italic> America). We cloned four representative proteins (one from the 120\u2005kDa and three from the 150\u2005kDa) that showed different epitope specificities, generated a chimera, and demonstrated that this regimen may bolster serodiagnostic reliability. Overall sensitivity and specificity, against sera from active\u2010\/mixed\u2010stage NC and those from other infections, and healthy\u2010controls, were determined to be 97.5% (156\/160 samples) and 97.8% (265\/271 cases). Patient sera from adult taeniases, sparganosis, and fascioliasis showed weak cross\u2010reactions. Micro\u2010ELISA showed similar results. This chimera may prove useful in the construction of standardized platform for NC serodiagnosis.<\/jats:p>","DOI":"10.1002\/prca.200800084","type":"journal-article","created":{"date-parts":[[2008,10,22]],"date-time":"2008-10-22T19:53:33Z","timestamp":1224705213000},"page":"1596-1610","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A recombinant chimeric antigen toward a standardized serodiagnosis of <b><i>Taenia solium<\/i><\/b> neurocysticercosis"],"prefix":"10.1002","volume":"2","author":[{"given":"Young\u2010An","family":"Bae","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon\u2010Tack","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joon\u2010Yong","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seon\u2010Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagadish","family":"Mahanta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chom\u2010Kyu","family":"Chong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong\u2010Soo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,2]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Neurocysticercosis: A Clinical Handbook","author":"Del Brutto O. 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