{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:49:40Z","timestamp":1790232580328,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,6,24]],"date-time":"2009-06-24T00:00:00Z","timestamp":1245801600000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ligue contre le cancer, Comit\u00e9 des Deux-S\u00e8vres, France"},{"name":"consortium INCA from the European Union"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    To date no reliable diagnostic method exists to predict, among the very large and clinically heterogeneous group of\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    \u2010infected patients, the extremely small group at risk for developing low\u2010grade gastric MALT lymphoma (LG\u2010MALT). Search of proteomic biomarkers holds promise for the classification of the\n                    <jats:italic>H. pylori<\/jats:italic>\n                    strains with regard to this severe clinical outcome. In the present study 69\n                    <jats:italic>H. pylori<\/jats:italic>\n                    strains isolated from patients with two different\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010associated diseases, duodenal ulcer (DU,\n                    <jats:italic>n<\/jats:italic>\n                    =29) and LG\u2010MALT (\n                    <jats:italic>n<\/jats:italic>\n                    =40) were used. Protein expression patterns of the strains were analyzed by using the high\u2010throughput methodology SELDI. Selected proteins were purified by means of chromatographic and electrophoretic methods in view of further sequencing by LC\u2010MS\/MS. Univariate analysis (Mann\u2013Whitney test) of the protein expression patterns generated nine significant biomarkers that can discriminate between\n                    <jats:italic>H. pylori<\/jats:italic>\n                    strains from patients with DU and LG\u2010MALT. These biomarkers are of low molecular weight, ranging from 6 to 26.6\u2009kDa. Among them, two are overexpressed in LG\u2010MALT strains and seven \u2013 in DU strains. Two biomarker proteins, one overexpressed in LG\u2010MALT strains (13.2\u2009kDa) and another one \u2013 overexpressed in DU strains (26.6\u2009kDa), were purified to homogeneity and identified by using LC\u2010MS\/MS as a 50S ribosomal protein L7\/L12 and a urease subunit, respectively. These biomarkers can be included in novel protein arrays for the differential diagnosis of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010associated clinical outcomes.\n                  <\/jats:p>","DOI":"10.1002\/prca.200800158","type":"journal-article","created":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:19:14Z","timestamp":1246407554000},"page":"672-681","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Proteomic\n                    <i>Helicobacter pylori<\/i>\n                    biomarkers discriminative of low\u2010grade gastric MALT lymphoma and duodenal ulcer"],"prefix":"10.1002","volume":"3","author":[{"given":"C\u00e9dric","family":"Bernarde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghalia","family":"Khoder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Lehours","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Burucoa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Louis","family":"Fauch\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Charles","family":"Delchier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"M\u00e9graud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christo","family":"Atanassov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,24]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1","article-title":"Schistosomes liver flukes and Helicobacter pylori. 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