{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T03:25:34Z","timestamp":1782444334963,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,8,18]],"date-time":"2009-08-18T00:00:00Z","timestamp":1250553600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Urinary proteomic profiling has potential to identify candidate biomarkers of renal injury in infants provided an adequate urine sample can be obtained. Although diapers are used to obtain urine for clinical evaluation, their use for proteomic analysis has not been investigated. We therefore performed feasibility studies on the use of diaper\u2010extracted urine for 2\u2010D PAGE. Pediatric waste urine (2\u201320\u2009mL) was applied to gel\u2010containing, non\u2010gel and cotton\u2010gauze diapers and then mechanically expressed. Urine volume and total protein were measured pre\u2010 and post\u2010extraction. Proteins were separated <jats:italic>via<\/jats:italic> 2\u2010D PAGE following application of urine (20\u201340\u2009mL) to each matrix. 2\u2010D PAGE was also performed on clinical specimens collected using each diaper type. Differences in the adsorption and retention of urine volume and protein were noted between matrices. Non\u2010gel and cotton\u2010gauze diapers provided the best protein\/volume recovery and the lowest interference with the Bradford assay. 2\u2010D PAGE was also successfully completed using urine samples from both cotton fiber matrices. Conversely, samples from low\u2010gel diapers demonstrated poor protein separation and reproducibility. Diapers containing cotton\u2013fiber matrices appear adequate for 2\u2010D PAGE. Qualitative and quantitative analyses of resolved proteins using replicate, high\u2010resolution gels will be required, however, before diaper\u2010extracted urine can be applied in proteomic profiling.<\/jats:p>","DOI":"10.1002\/prca.200900045","type":"journal-article","created":{"date-parts":[[2009,8,18]],"date-time":"2009-08-18T12:50:44Z","timestamp":1250599844000},"page":"989-999","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Urine collected from diapers can be used for 2\u2010D PAGE in infants and young children"],"prefix":"10.1002","volume":"3","author":[{"given":"Mary Jayne","family":"Kennedy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Griffin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifeng","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Merchant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,8,18]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"405A","article-title":"Urinary proteomic profiling identifies differentially expressed proteins in aminoglycoside\u2010treated children with and without nephrotoxicity","volume":"17","author":"Kennedy M. 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