{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:36:03Z","timestamp":1789187763487,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T00:00:00Z","timestamp":1267142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Purpose<\/jats:bold>: We determined the potential value of protein profiling of tissue samples by assessing how precise this approach enables discrimination of B\u2010cell lymphoma from reactive lymph nodes, and how well the profiles can be used for lymphoma classification.<\/jats:p><jats:p><jats:bold>Experimental design<\/jats:bold>: Protein lysates from lymph nodes (<jats:italic>n<\/jats:italic>=239) from patients with the diagnosis of reactive hyperplasia (<jats:italic>n<\/jats:italic>=44), follicular lymphoma (<jats:italic>n<\/jats:italic>=63), diffuse large B\u2010cell lymphoma (<jats:italic>n<\/jats:italic>=43), mantle cell lymphoma (<jats:italic>n<\/jats:italic>=47), and chronic lymphocytic leukemia\/small lymphocytic B\u2010cell lymphoma (<jats:italic>n<\/jats:italic>=42) were analysed by SELDI\u2010TOF MS. Data analysis was performed by (i) classification and regression tree\u2010based analysis and (ii) binary and polytomous logistic regression analysis.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold>: After internal validation by the leave\u2010one\u2010out principle, both the classification and regression tree and logistic regression classification correctly identified the majority of the malignant (87 and 96%, respectively) and benign cases (73 and 75%, respectively). Classification was less successful since approximately one\u2010third of the cases of each group were misclassified according to the histological classification. However, an additional mantle cell lymphoma case that was misclassified as chronic lymphocytic leukemia\/small lymphocytic B\u2010cell lymphoma initially was identified based on the protein profile.<\/jats:p><jats:p><jats:bold>Conclusions and clinical relevance<\/jats:bold>: SELDI\u2010TOF MS protein profiling allows for reliable identification of the majority of malignant lymphoma cases; however, further validation and testing robustness in a diagnostic setting is needed.<\/jats:p>","DOI":"10.1002\/prca.200900120","type":"journal-article","created":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T23:08:56Z","timestamp":1267225736000},"page":"519-527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Protein profiling in pathology: Analysis and evaluation of 239 frozen tissue biopsies for diagnosis of B\u2010cell lymphomas"],"prefix":"10.1002","volume":"4","author":[{"given":"Corine","family":"Jansen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ton","family":"Feuth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M. M.","family":"Raemaekers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos","family":"Rijntjes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos W.","family":"Meijer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter J.","family":"Westenend","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joop","family":"van Baarlen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan H. J. M.","family":"van Krieken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konnie M.","family":"Hebeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia J. T. A.","family":"Groenen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,4,29]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2002.6678"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500472"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209770"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr050349r"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603188"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-0586"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2005.07.033"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1309\/UEDXPHAAP740B61D"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800100"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr060058y"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.21581"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-7-235"},{"key":"e_1_2_7_14_2","first-page":"27","article-title":"Protein profiling of B\u2010cell lymphomas using tissue biopsies: A potential tool for small samples in pathology","volume":"30","author":"Jansen C.","year":"2008","journal-title":"Cell Oncol."},{"key":"e_1_2_7_15_2","article-title":"Code for proper secondary use of human tissue in The Netherlands","author":"Federation of medical Scientific Societies","year":"2002","journal-title":"Federa"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-3462-1"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm486"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055351"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21500"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa041869"}],"container-title":["PROTEOMICS \u2013 Clinical Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fprca.200900120","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fprca.200900120","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.200900120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T07:09:27Z","timestamp":1697440167000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.200900120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,29]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1002\/prca.200900120"],"URL":"https:\/\/doi.org\/10.1002\/prca.200900120","archive":["Portico"],"relation":{},"ISSN":["1862-8346","1862-8354"],"issn-type":[{"value":"1862-8346","type":"print"},{"value":"1862-8354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,29]]},"assertion":[{"value":"2009-06-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-04-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}