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In this review, we will cover technical aspects of CE\u2010MS coupling and highlight the improvements made in the last few years. We examine CE\u2010MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. We discus the principal theoretical and practical obstacles encountered when employing CE\u2010MS (and most other proteomic technologies) for the analysis of body fluids for biomarker discovery. We will present several examples of a successful application of CE\u2010MS for biomarker discovery, implications for disease diagnosis, prognosis, and therapy evaluation, and will discuss current challenges and possible future improvements.<\/jats:p>","DOI":"10.1002\/prca.201000058","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T14:12:27Z","timestamp":1286287947000},"page":"9-23","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["CE\u2010MS in biomarker discovery, validation, and clinical application"],"prefix":"10.1002","volume":"5","author":[{"given":"Harald","family":"Mischak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost 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