{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:44:50Z","timestamp":1740145490270,"version":"3.37.3"},"reference-count":51,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2012,8,28]],"date-time":"2012-08-28T00:00:00Z","timestamp":1346112000000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"Scientific and Technological Research Council of Turkey","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Sodium dodecyl sulphate\u2010polyacrylamide gel electrophoresis (1<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content>) may reveal qualitative or quantitative defects in red blood cell (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>) membrane proteins, two\u2010dimensional gel electrophoresis (2<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content>) can be used for determination of the protein changes caused by the disease process in a relatively high\u2010throughput manner, because it permits an analysis of thousands of modified or unmodified proteins simultaneously. The principal aim of this study was to compare hereditary elliptocytosis (<jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>), hereditary spherocytosis (<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>), and control <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> membrane protein profiles and identify proteins as a clinical marker by the sensitive methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> membrane proteins of <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>, and control groups were compared by 2<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content> and matrix\u2010assisted laser desorption\/ionization\u2010time of flight (<jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> analysis was obtained using peptide mass fingerprint for protein identification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty proteins were identified with peptide mass fingerprint analysis and different expression levels were found in <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>, and controls for some proteins that includes three biomarker proteins (ankyrin, spectrin, band 3) that may have prognostic or predictive importance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>2DE of <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> proteins is a potentially valuable method for studying heritable disorders such as <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>. By identifying a deficiency in membrane proteins associated with the <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> cytoskeleton, the diagnosis of <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> can be confirmed.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201200010","type":"journal-article","created":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T06:15:05Z","timestamp":1342678505000},"page":"403-411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comprehensive identification of erythrocyte membrane protein deficiency by 2<scp>D<\/scp> gel electrophoresis based proteomic analysis in hereditary elliptocytosis and spherocytosis"],"prefix":"10.1002","volume":"6","author":[{"given":"Duygu Ozel","family":"Demiralp","sequence":"first","affiliation":[{"name":"Ankara University Biotechnology Institute  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selen","family":"Peker","sequence":"additional","affiliation":[{"name":"Ankara University Biotechnology Institute  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burhan","family":"Turgut","sequence":"additional","affiliation":[{"name":"Department of Child Health and Diseases TOBB University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nejat","family":"Akar","sequence":"additional","affiliation":[{"name":"Department of Hematology, Medical School Namik Kemal University  Tekirdag Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,8,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07091.x"},{"key":"e_1_2_7_3_1","first-page":"249","article-title":"Clinical expression and laboratory detection of red blood cell membrane protein mutations","volume":"30","author":"Palek J.","year":"1993","journal-title":"Semin. 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