{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:43:46Z","timestamp":1791269026976,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2013,11,4]],"date-time":"2013-11-04T00:00:00Z","timestamp":1383523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Science and Technology Commission of Shanghai Municipality","award":["10JC1409900"],"award-info":[{"award-number":["10JC1409900"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31230048\/c120101"],"award-info":[{"award-number":["31230048\/c120101"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Ministry of Science and Technology of China Grants 973 program","award":["2011CB944304"],"award-info":[{"award-number":["2011CB944304"]}]},{"name":"The Ministry of Science and Technology of China Grants 973 program","award":["2011CB944504"],"award-info":[{"award-number":["2011CB944504"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>To identify biochemical markers in men with idiopathic infertility and normal sperm counts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>We obtained proteomic profiling proteins in human spermatozoa following successful or unsuccessful pregnancy via assisted reproductive technique (ART) using 6\u2010plex tandem mass tag (TMT) isobaric MS. Our study design consisted of two groups: (i) The semen of six men whose sperm resulted in a clinical pregnancy following ART and (ii) six men whose semen did not result in a clinical pregnancy following ART. The results of differentiated MS were validated by Western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and discussion<\/jats:title><jats:p>A total of 2045 proteins were detected in our cohort. Twenty\u2010one proteins were found to be differentially expressed (&gt;1.2\u2010fold) between Group 1 and Group 2. Using the results of bioinformatics analysis and Western blotting, three proteins (A2LD1, ATP1B3, and FBXO2) were shown to have the same differential pattern (<jats:italic>p<\/jats:italic> &lt; 0.05) that was observed in the MS analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>Proteomics may help identity a select cohort of men with normal semen parameters and aid infertility diagnoses.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201200078","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:37:39Z","timestamp":1381541859000},"page":"850-858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Differential proteomic profiling in human spermatozoa that did or did not result in pregnancy via IVF and AID"],"prefix":"10.1002","volume":"7","author":[{"given":"Yong","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Urology Sperm Development and Genetics Laboratory Renji Hospital Shanghai Human Sperm Bank Shanghai Institute of Andrology Shanghai Jiao Tong University School of Medicine  Shanghai P. 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