{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T15:59:18Z","timestamp":1792166358658,"version":"4.4.0"},"reference-count":23,"publisher":"Wiley","issue":"5-6","license":[{"start":{"date-parts":[[2013,5,17]],"date-time":"2013-05-17T00:00:00Z","timestamp":1368748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Clinical application of biomarker candidates discovered by proteomic analysis is challenging. The purpose of this study was to standardize preanalytical conditions for measurement of serum levels of fibrinogen alpha <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2010chain 5.9 kDa fragment (<jats:styled-content style=\"fixed-case\">FIC<\/jats:styled-content> 5.9) and to test the diagnostic value of this peptide for detection of early hepatic fibrosis in patients with hepatitis <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>)\u2010related chronic hepatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>Serum <jats:styled-content style=\"fixed-case\">FIC<\/jats:styled-content> 5.9 levels were measured by a sandwich <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Effects on the serum <jats:styled-content style=\"fixed-case\">FIC<\/jats:styled-content> 5.9 level of temperature, the time between venipuncture and serum separation, and the types of collection tubes used were examined. The diagnostic value of serum <jats:styled-content style=\"fixed-case\">FIC<\/jats:styled-content> 5.9 as an early indicator of hepatic fibrosis due to <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> was then assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">FIC<\/jats:styled-content> 5.9 was produced in a time\u2010 and temperature\u2010dependent manner after venipuncture. Abnormal <jats:styled-content style=\"fixed-case\">FIC<\/jats:styled-content> 5.9 values were found in 89.5% of <jats:styled-content style=\"fixed-case\">FI<\/jats:styled-content> stage patients. Receiver operating characteristic analyses confirmed the superiority of <jats:styled-content style=\"fixed-case\">FIC<\/jats:styled-content> 5.9 over other conventional markers for early detection of fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>The serum <jats:styled-content style=\"fixed-case\">FIC<\/jats:styled-content> 5.9 level may be an early indicator of hepatic fibrosis in <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010related chronic liver diseases. This study provides an example of a pipeline from biomarker discovery by proteome analysis to assay optimization and preliminary clinical validation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201200094","type":"journal-article","created":{"date-parts":[[2013,2,4]],"date-time":"2013-02-04T16:27:57Z","timestamp":1359995277000},"page":"424-431","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Serum fibrinogen alpha <scp>C<\/scp>\u2010chain 5.9 k<scp>D<\/scp>a fragment as a biomarker for early detection of hepatic fibrosis related to hepatitis <scp>C<\/scp> virus"],"prefix":"10.1002","volume":"7","author":[{"given":"Kazuyuki","family":"Sogawa","sequence":"first","affiliation":[{"name":"Clinical Proteomics Research Center Chiba University Hospital Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenta","family":"Noda","sequence":"additional","affiliation":[{"name":"Department of Molecular 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