{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:24:32Z","timestamp":1758817472837},"reference-count":45,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2015,9,8]],"date-time":"2015-09-08T00:00:00Z","timestamp":1441670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Ventricular septal defect (VSD) has intracardiac left\u2010to\u2010right shunt and increased pulmonary flow that may affect the acute phase response (APR). We examined the hypothesis that plasma proteins of VSD patients may be altered.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>2DE and MS were used to detect differential plasma proteins in VSD patients (<jats:italic>n<\/jats:italic> = 55) and controls (<jats:italic>n<\/jats:italic> = 70). Candidate APR proteins were confirmed by ELISA in new samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among three differentially expressed APR proteins from 322 protein spots detected, haptoglobin (0.4 \u00b1 0.04 versus 0.6 \u00b1 0.07 mg\/mL; <jats:italic>p<\/jats:italic> = 0.016) and serum amyloid P\u2010component (SAP) (3.8 \u00b1 0.2 versus 6.3 \u00b1 0.8 ng\/mL; <jats:italic>p<\/jats:italic> = 0.003) were significantly lower and orosomucoid 2 (3.1 \u00b1 0.1 mg\/mL versus 2.3 \u00b1 0.1 mg\/mL; <jats:italic>p<\/jats:italic> &lt; 0.001) was significantly higher in VSD patients than in normal controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>The plasma concentration of three acute phase proteins, haptoglobin, SAP, and orosomucoid 2 are altered that may reflect inflammation, be associated with decreased innate immune system function, and predispose the VSD patients to vulnerability to infections and pulmonary disease. These three proteins in plasma may also be developed as biomarkers for the function of innate immune system in patients with congenital heart disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201400166","type":"journal-article","created":{"date-parts":[[2015,4,25]],"date-time":"2015-04-25T10:51:16Z","timestamp":1429959076000},"page":"1087-1096","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Acute phase proteins altered in the plasma of patients with congenital ventricular septal defect"],"prefix":"10.1002","volume":"9","author":[{"given":"Xi","family":"Zhang","sequence":"first","affiliation":[{"name":"TEDA International Cardiovascular Hospital Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery Tianjin First Center Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Yang","sequence":"additional","affiliation":[{"name":"TEDA International Cardiovascular Hospital Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University  Hangzhou China"},{"name":"Department of Medicine and Therapeutics The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA International Cardiovascular Hospital Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Xuan","sequence":"additional","affiliation":[{"name":"TEDA International Cardiovascular Hospital Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Cheng","family":"Liu","sequence":"additional","affiliation":[{"name":"TEDA International Cardiovascular Hospital Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"TEDA International Cardiovascular Hospital Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo\u2010Wei","family":"He","sequence":"additional","affiliation":[{"name":"TEDA International Cardiovascular Hospital Tianjin and The Affiliated Hospital of Hangzhou Normal University and Zhejiang University  Hangzhou China"},{"name":"Department of Surgery Oregon Health and Science University  Portland Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(02)01886-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.618124"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.191259"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.05116.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00246-010-9813-7"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.714402"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.592410"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1586\/erc.09.153"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.06.004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01827"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5373.108"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0197-30"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/gtmb.2012.0435"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/gtmb.2012.0003"},{"key":"e_1_2_8_16_1","first-page":"295","article-title":"Immunological profile in congenital heart disease","volume":"31","author":"Khalil A.","year":"1994","journal-title":"Indian Pediatr."},{"key":"e_1_2_8_17_1","first-page":"143","article-title":"Prevalence of t\u2010lymphocyte abnormalities in infants with congenital heart disease","volume":"138","author":"Kiel E. 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