{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:06:40Z","timestamp":1792782400196,"version":"4.5.0"},"reference-count":63,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2015,6,12]],"date-time":"2015-06-12T00:00:00Z","timestamp":1434067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AR063124"],"award-info":[{"award-number":["AR063124"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AI103980"],"award-info":[{"award-number":["AI103980"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AI24717"],"award-info":[{"award-number":["AI24717"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["TR000077"],"award-info":[{"award-number":["TR000077"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["HG006828"],"award-info":[{"award-number":["HG006828"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Juvenile Diabetes Research Foundation international","award":["1-2011-588"],"award-info":[{"award-number":["1-2011-588"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Patients with systemic lupus erythematosus (SLE) frequently develop lupus nephritis (LN), a complication frequently leading to end stage kidney disease. Immune complex deposition in the glomerulus is central to the development of LN. Using a targeted proteomic approach, we tested the hypothesis that autoantibodies targeting glomerular antigens contribute to the development of LN.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental design<\/jats:title>\n                    <jats:p>Human podocyte and glomerular proteins were separated by SDS\u2010PAGE and immunoblotted with sera from SLE patients with and without LN. The regions of those gels corresponding to reactive bands observed with sera from LN patients were analyzed using LC\u2010MS\/MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>LN reactive bands were seen at approximately 50 kDa in podocyte extracts and between 36 and 50 kDa in glomerular extracts. Those bands were analyzed by LC\u2010MS\/MS and 102 overlapping proteins were identified. Bioinformatic analysis determined that 36 of those proteins were membrane associated, including a protein previously suggested to contribute to glomerulonephritis and LN, annexin A2. By ELISA, patients with proliferative LN demonstrated significantly increased antibodies against annexin A2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion and clinical relevance<\/jats:title>\n                    <jats:p>Proteomic approaches identified multiple candidate antigens for autoantibodies in patients with LN. Serum antibodies against annexin A2 were significantly elevated in subjects with proliferative LN, validating those antibodies as potential biomarkers.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.201400175","type":"journal-article","created":{"date-parts":[[2015,3,30]],"date-time":"2015-03-30T18:35:44Z","timestamp":1427740544000},"page":"1012-1020","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Autoantibodies targeting glomerular annexin A2 identify patients with proliferative lupus nephritis"],"prefix":"10.1002","volume":"9","author":[{"given":"Dawn J.","family":"Caster","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Louisville School of Medicine  Louisville KY USA"},{"name":"Robley Rex Veterans Affairs Medical Center  Louisville KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik A.","family":"Korte","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Louisville School of Medicine  Louisville KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Merchant","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Louisville School of Medicine  Louisville KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon B.","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Louisville School of Medicine  Louisville KY USA"},{"name":"Robley Rex Veterans Affairs Medical Center  Louisville KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel W.","family":"Wilkey","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Louisville School of Medicine  Louisville KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brad H.","family":"Rovin","sequence":"additional","affiliation":[{"name":"Department of Medicine the Ohio State University  Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan J.","family":"Birmingham","sequence":"additional","affiliation":[{"name":"Department of Medicine the Ohio State University  Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John B.","family":"Harley","sequence":"additional","affiliation":[{"name":"Center for Autoimmune Genomics and Etiology Department of Pediatrics Cincinnati Children's Hospital Medical Center and the University of Cincinnati  Cincinnati OH USA"},{"name":"US Department of Veterans Affairs Medical Center  Cincinnati OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beth L.","family":"Cobb","sequence":"additional","affiliation":[{"name":"Center for Autoimmune Genomics and Etiology Department of Pediatrics Cincinnati Children's Hospital Medical Center and the University of Cincinnati  Cincinnati OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahram","family":"Namjou","sequence":"additional","affiliation":[{"name":"Center for Autoimmune Genomics and Etiology Department of Pediatrics Cincinnati Children's Hospital Medical Center and the University of Cincinnati  Cincinnati OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth R.","family":"McLeish","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Louisville School of Medicine  Louisville KY USA"},{"name":"Robley Rex Veterans Affairs Medical Center  Louisville KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Powell","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Louisville School of Medicine  Louisville KY USA"},{"name":"Department of Biochemistry and Molecular Biology University of Louisville School of Medicine  Louisville KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1100359"},{"key":"e_1_2_8_3_1","first-page":"321359","article-title":"Serology of Lupus erythematosus: correlation between immunopathological features and clinical aspects","volume":"2014","author":"Cozzani E.","year":"2014","journal-title":"Autoimmune Dis."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e3181c9529c"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00443.x"},{"key":"e_1_2_8_6_1","first-page":"1495","article-title":"Multiple autoantibodies form the glomerular immune deposits in patients with systemic lupus erythematosus","volume":"30","author":"Mannik M.","year":"2003","journal-title":"J. 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