{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:28:25Z","timestamp":1790270905529,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"5-6","license":[{"start":{"date-parts":[[2015,5,15]],"date-time":"2015-05-15T00:00:00Z","timestamp":1431648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Nephrology Society"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Recent findings indicate that urinary extracellular vesicles (EVs) might reflect the pathophysiological state of urinary system; and that EVs\u2010induced ciliary signaling is a possible mechanism of intercellular communication within the tract. Here, we aimed to analyze the protein expression of urinary EVs during autosomal dominant polycystic kidney disease (ADPKD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>EVs were isolated from pooled urine samples of healthy control and ADPKD patients at two different stages of the disease and under tolvaptan treatment using the double\u2010cushion ultracentrifugation method. Proteins were identified and quantified by iTRAQ and multidimensional protein identification technology (MudPIT)\u2010based quantitative proteomics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Quantitative analyses identified 83 differentially expressed EV proteins. Many of these have apical membrane origin and are involved in signal transduction pathways of primary cilia, Ca<jats:sup>2+<\/jats:sup>\u2010activated signaling, cell\u2010cycle regulation, and cell differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>The reduced AQP\u20102 and the increased APO\u2010A1 levels observed in all ADPKD\u2010affected groups may reflects the impaired renal concentrating capability of these patients and correlated with estimated glomerular filtration rate decline. The levels of some upregulated proteins involved in Ca<jats:sup>2+<\/jats:sup>\u2010activated signaling declined upon tolvaptan treatment. The results obtained suggest that the quantitative proteomics of urinary EVs might be useful to monitor proteins difficult to access noninvasively, and thus advance our understanding of urinary tract physiology and pathology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201400199","type":"journal-article","created":{"date-parts":[[2015,3,9]],"date-time":"2015-03-09T06:31:29Z","timestamp":1425882689000},"page":"552-567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Urinary extracellular vesicles as reservoirs of altered proteins during the pathogenesis of polycystic kidney disease"],"prefix":"10.1002","volume":"9","author":[{"given":"Gabriella","family":"Pocsfalvi","sequence":"first","affiliation":[{"name":"Mass Spectrometry and Proteomics Institute of Biosciences and BioResources National Research Council of Italy  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delfin A. A.","family":"Raj","sequence":"additional","affiliation":[{"name":"Mass Spectrometry and Proteomics Institute of Biosciences and BioResources National Research Council of Italy  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Immacolata","family":"Fiume","sequence":"additional","affiliation":[{"name":"Mass Spectrometry and Proteomics Institute of Biosciences and BioResources National Research Council of Italy  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Vilasi","sequence":"additional","affiliation":[{"name":"Mass Spectrometry and Proteomics Institute of Biosciences and BioResources National Research Council of Italy  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Trepiccione","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Cardio\u2010Vascular Sciences Second University of Naples  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovambattista","family":"Capasso","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Cardio\u2010Vascular Sciences Second University of Naples  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2014.47.10.164"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-011-0689-3"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2012.12.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200329"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.19"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.5410\/wjcu.v3.i2.66"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201100477"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2008011"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.01.031"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008060564"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00093.2010"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.220277"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2011.04.012"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1205511"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800485"},{"key":"e_1_2_8_17_1","unstructured":"Raj D. 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