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Candidate protein expression was confirmed in hamster models and human CCA tissue microarray (TMA) using immunohistochemistry and Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Proteomics analysis detected 14\u20103\u20103 eta only in infected hamsters, not in uninfected controls. Immunohistochemistry and Western blot analysis confirmed low expression of 14\u20103\u20103 eta in normal hamster livers and demonstrated increased expression through time in infected livers. This protein was also observed in parasite organs, especially during the chronic phase of opisthorchiasis. Moreover, increased expression of 14\u20103\u20103 eta, relative to normal hamster livers, was observed during the early stage of CCA induced by OV infection and administration of <jats:italic>N<\/jats:italic>\u2010nitrosodimethylamine. Immunohistochemical analysis of human TMA revealed that 14\u20103\u20103 eta was highly expressed in CCA (84.23%, 187\/222 cases) but was not found in hepatocellular carcinoma or healthy liver tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>14\u20103\u20103 eta protein has potential as a screening and early diagnostic marker for CCA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201500019","type":"journal-article","created":{"date-parts":[[2015,10,5]],"date-time":"2015-10-05T10:26:48Z","timestamp":1444040808000},"page":"248-256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Upregulation of <b>14\u20103\u20103<\/b> eta in chronic liver fluke infection is a potential diagnostic marker of cholangiocarcinoma"],"prefix":"10.1002","volume":"10","author":[{"given":"Ornuma","family":"Haonon","sequence":"first","affiliation":[{"name":"Department of Parasitology Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rucksak","family":"Rucksaken","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Porntip","family":"Pinlaor","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Centre for Research and Development in Medical Diagnostic Laboratory Faculty of Associated Medical Sciences, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chawalit","family":"Pairojkul","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Department of Pathology Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaovalux","family":"Chamgramol","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Department of Pathology Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kitti","family":"Intuyod","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Biomedical Science Program Graduate School, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudarat","family":"Onsurathum","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narong","family":"Khuntikeo","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Department of Surgery Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somchai","family":"Pinlaor","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine, Khon Kaen University  Khon Kaen Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2012.07.006"},{"key":"e_1_2_8_3_1","first-page":"341","article-title":"Review of human carcinogens: Opisthorchis viverrini and Clonorchis sinensis","volume":"100","author":"IARC, A.","year":"2012","journal-title":"IARC Monographs on the Evaluation of Carcinogenic Risks to Humans"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040201"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.08.014"},{"key":"e_1_2_8_6_1","first-page":"193","article-title":"Cholangiocarcinoma in north east Thailand. 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