{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:06:07Z","timestamp":1753869967574,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T00:00:00Z","timestamp":1482278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["NSC 102\u20102314\u2010B\u2010303\u2010014"],"award-info":[{"award-number":["NSC 102\u20102314\u2010B\u2010303\u2010014"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Ketamine\u2010induced cystitis (KC) among chronic ketamine young abusers has increased dramatically and it has brought attention for Urologists. The underlying pathophysiological mechanism(s) of KC is still unclear. Therefore, the purpose of this study is to elucidate the possible pathophysiological mechanism(s) of KC through proteomic techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>Bladder tissues are obtained from seven patients with KC, seven patients with interstitial cystitis\/bladder pain syndrome, and five control subjects who underwent video\u2010urodynamic study followed by augmentation enterocystoplasty to increase bladder capacity. 2DE\/MS\/MS\u2010based approach, functional classifications, and network analyses are used for proteomic and bioinformatics analyses and protein validation is carried out by Western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the proteins identified, bioinformatics analyses revealed that several actin binding related proteins such as cofilin\u20101, myosin light polypeptide 9, filamin A, gelsolin, lamin A are involved in the apoptosis. Besides, the contractile proteins and cytoskeleton proteins such as myosin light polypeptide 9, filamin A, and calponin are found downregulated in KC bladders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>Increased apoptosis in KC might be mediated by actin\u2010binding proteins and a Ca<jats:sup>2+<\/jats:sup>\u2010activated protease. Rapid detrusor contraction in KC might be induced by contractile proteins and cytoskeleton proteins.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201600085","type":"journal-article","created":{"date-parts":[[2016,11,10]],"date-time":"2016-11-10T14:15:51Z","timestamp":1478787351000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The putative involvement of actin\u2010binding proteins and cytoskeleton proteins in pathological mechanisms of ketamine cystitis\u2014Revealed by a prospective pilot study using proteomic approaches"],"prefix":"10.1002","volume":"11","author":[{"given":"Hsueh\u2010Hui","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Medical Research Buddhist Tzu Chi General Hospital  Hualien 970 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Jun","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Medical Research Buddhist Tzu Chi General Hospital  Hualien 970 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hann\u2010Chorng","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Urology Buddhist Tzu Chi General Hospital, Tzu Chi University  Hualien 970 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,12,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2007.01.038"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2008.07920.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.12256"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.04.102"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/2051-5960-1-64"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.22367"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-014-3616-5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00607.2014"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/iju.12100"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2009.02326.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20798"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.05.045"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-175-2_7"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"D358","DOI":"10.1093\/nar\/gkl825","article-title":"STRING 7\u2010recent developments in the integration and prediction of protein interactions","volume":"35","author":"Mering C.","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1320815111"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5336.294"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47193-0"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.108.531632"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)67106-6"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(97)01085-2"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.20.3771"},{"key":"e_1_2_8_23_1","first-page":"65","article-title":"Role of calpain in apoptosis","volume":"13","author":"Momeni H. 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