{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:27:05Z","timestamp":1780471625893,"version":"3.54.1"},"reference-count":36,"publisher":"Wiley","issue":"5-6","license":[{"start":{"date-parts":[[2017,1,17]],"date-time":"2017-01-17T00:00:00Z","timestamp":1484611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000980","name":"Arthritis Foundation","doi-asserted-by":"publisher","award":["Innovative Research Grant"],"award-info":[{"award-number":["Innovative Research Grant"]}],"id":[{"id":"10.13039\/100000980","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007253","name":"Nancy Taylor Foundation for Chronic Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007253","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["NIH 1K23AR066724\u201001"],"award-info":[{"award-number":["NIH 1K23AR066724\u201001"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["NIH 8P20GM103464"],"award-info":[{"award-number":["NIH 8P20GM103464"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["P20 RR020173"],"award-info":[{"award-number":["P20 RR020173"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>The goal is to investigate the specific contribution of fibroblast\u2010like synoviocytes (FLS) to the inflammatory milieu of the synovium in juvenile idiopathic arthritis (JIA) through detection of secreted proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>Expression of 89 cytokines and chemokines is determined on unprocessed synovial fluid from controls and JIA patients using antibody arrays. Supernatants from pure cell cultures of FLS grown from synovial fluids or tissues from JIA and controls are also examined for protein expression. Ingenuity Pathway Analysis (IPA) is revealed top pathways and upstream regulators of significant proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Protein studies is revealed that JIA FLS release pro\u2010inflammatory cytokines and chemokines, including IL\u20104, IL\u20106, IL\u201017, CXCL1, and CXCL6, and lose expression of important regulator signals, such as IL\u201010 and TIMP2. Of the 84 proteins differentially expressed between controls and JIA in the synovial fluid, 1\/3 (29 proteins) are differentially expressed in the cell culture supernatants of JIA and control FLS. ELISA of cell culture supernatants and synovial fluid confirmed seven key proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and clinical relevance<\/jats:title><jats:p>JIA FLS are central to perpetuation of inflammation in JIA, including trafficking of inflammatory cells and effects on the extracellular matrix. These cells express key disease\u2010specific chemokines that, with further refinement, may allow us to tailor therapy appropriately.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201600088","type":"journal-article","created":{"date-parts":[[2016,12,25]],"date-time":"2016-12-25T04:52:32Z","timestamp":1482641552000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Secretion of pro\u2010inflammatory cytokines and chemokines and loss of regulatory signals by fibroblast\u2010like synoviocytes in juvenile idiopathic arthritis"],"prefix":"10.1002","volume":"11","author":[{"given":"AnneMarie C.","family":"Brescia","sequence":"first","affiliation":[{"name":"Nemours\/Thomas Jefferson University Wilmington DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan M.","family":"Simonds","sequence":"additional","affiliation":[{"name":"Nemours Biomedical Research Wilmington DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen E.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Pennsylvania Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos D.","family":"Rose","sequence":"additional","affiliation":[{"name":"Nemours\/Thomas Jefferson University Wilmington DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199805)41:5<778::AID-ART4>3.0.CO;2-V"},{"key":"e_1_2_8_3_1","first-page":"1520","article-title":"Worldwide prevalence of juvenile arthritis why does it vary so much","volume":"29","author":"Manners P. 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