{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:32:34Z","timestamp":1790616754302,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"9-10","license":[{"start":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:00:00Z","timestamp":1495497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Dr. Izador I. Cabakoff Research Endowment Fund, the Hebrew University - Hadassah School of Dental Medicine, Jerusalem, Israel","award":["0364762"],"award-info":[{"award-number":["0364762"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Orthodontically induced inflammatory root resorption (OIIRR) is one of the most prevalent and unavoidable consequence of orthodontic tooth movement. The aim of this study was to discover potential diagnostic protein biomarkers for detection of OIIRR in whole saliva (WS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Unstimulated WS was collected from 72 subjects: 48 OIIRR patients and 24 untreated, generally healthy, age and gender matched controls. Radiographic assessment of periapical x\u2010rays of four upper incisors taken before and 9 months after bonding was done. High\u2010abundance proteins were depleted followed by two\u2010dimensional\u2010gel\u2010electrophoresis and quantitative mass spectrometry (qMS). Finally, to initially validate qMS results, Western blotting was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>qMS revealed differentially expressed proteins in the moderate\u2010to\u2010severe OIIRR group, which have never been found in WS before. Additionally, in the moderate\u2010to\u2010severe young OIIRR group, the pathogenetic mechanisms related to actin cytoskeleton regulation and Fc gamma R\u2010 mediated phagocytosis were detected, while in adults\u2010 to focal adhesion. Preliminary validation by Western blotting of fetuin\u2010A and p21\u2010ARC indicated expression profile trends similar to those identified by qMS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The significance of WS novel proteomic methodologies is clearly demonstrated for detecting new OIIRR biomarkers as well as for unveiling possible novel pathogenetic mechanisms in both young and adult patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201600119","type":"journal-article","created":{"date-parts":[[2017,3,30]],"date-time":"2017-03-30T01:26:42Z","timestamp":1490837202000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Identification of salivary protein biomarkers for orthodontically induced inflammatory root resorption"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9243-3652","authenticated-orcid":false,"given":"Karolina El\u017cbieta","family":"Kaczor\u2010Urbanowicz","sequence":"first","affiliation":[{"name":"Department of Orthodontics The Hebrew University of Jerusalem Hadassah School of Dental Medicine  Israel"},{"name":"Institute of Dental Sciences Faculty of Dental Medicine The Hebrew University of Jerusalem  Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer","family":"Deutsch","sequence":"additional","affiliation":[{"name":"Institute of Dental Sciences Faculty of Dental Medicine The Hebrew University of Jerusalem  Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Batia","family":"Zaks","sequence":"additional","affiliation":[{"name":"Institute of Dental Sciences Faculty of Dental Medicine The Hebrew University of Jerusalem  Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Krief","sequence":"additional","affiliation":[{"name":"Institute of Dental Sciences Faculty of Dental Medicine The Hebrew University of Jerusalem  Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella","family":"Chaushu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics The Hebrew University of Jerusalem Hadassah School of Dental Medicine  Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Palmon","sequence":"additional","affiliation":[{"name":"Institute of Dental Sciences Faculty of Dental Medicine The Hebrew University of Jerusalem  Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,23]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","first-page":"52","article-title":"Preliminary evaluation of morphological parameters of the saliva in patients undergoing orthodontic treatment","volume":"51","author":"Kasacka I.","year":"2006","journal-title":"Adv. Med. Sci."},{"key":"e_1_2_9_3_1","first-page":"175","article-title":"Orthodontically induced inflammatory root resorption. Part. I: the Basic science aspects","volume":"72","author":"Brezniak N.","year":"2002","journal-title":"Angle. Orthod."},{"key":"e_1_2_9_4_1","first-page":"231","article-title":"A study of root resorption in treated Class division 1 malocclusions","volume":"39","author":"Deshields R.","year":"1969","journal-title":"Angle. Orthod."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-5406(96)80015-1"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/2.4.197-a"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-5406(05)81678-6"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/10.1.30"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/16.3.223"},{"key":"e_1_2_9_10_1","first-page":"180","article-title":"Orthodontically induced inflammatory root resorption. Part. II: The clinical aspects","volume":"72","author":"Brezniak N.","year":"2002","journal-title":"Angle. Orthod."},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(00)00088-1"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345960750010801"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(96)00063-5"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(94)90165-1"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ortho\/28.4.281"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-5406(94)70131-8"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-5406(96)70152-X"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1067\/mod.2002.121011"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cjq145"},{"key":"e_1_2_9_20_1","first-page":"201","volume-title":"Biological Mechanisms of Tooth Movement and Craniofacial Adaptation","author":"Evans C. A.","year":"2000"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/26.1.25"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2006.08.018"},{"key":"e_1_2_9_23_1","first-page":"1338","article-title":"Detection of dentin proteins in saliva. Implication as root resorption biomarkers","volume":"92","author":"Gyllenhaal K.","year":"2013","journal-title":"J. Dent. Res."},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201100655"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200800207"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2010.01700.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.05.012"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keu405"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2008.01465.x"},{"key":"e_1_2_9_30_1","volume-title":"Infectious Dis. Children","author":"Gershon A. A.","year":"2004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cjl001"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.72.6.1092"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.7.1171"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-66-1-187"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3280105"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0804439"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.037168"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2014.03.013"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.0301238"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1100\/2012\/647240"}],"container-title":["PROTEOMICS \u2013 Clinical Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fprca.201600119","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.201600119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T05:11:44Z","timestamp":1696482704000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.201600119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,23]]},"references-count":39,"journal-issue":{"issue":"9-10","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/prca.201600119"],"URL":"https:\/\/doi.org\/10.1002\/prca.201600119","archive":["Portico"],"relation":{},"ISSN":["1862-8346","1862-8354"],"issn-type":[{"value":"1862-8346","type":"print"},{"value":"1862-8354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,23]]},"assertion":[{"value":"2016-08-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-03-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-05-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1600119"}}