{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:30:15Z","timestamp":1761611415064,"version":"3.41.2"},"reference-count":36,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2017,4,3]],"date-time":"2017-04-03T00:00:00Z","timestamp":1491177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Apolipoprotein A\u2010I (ApoA\u2010I) and apolipoprotein B\u2010100 (ApoB\u2010100) are amphipathic proteins that are strong predictors of cardiovascular disease risk. The traceable calibration of apolipoprotein assays is a persistent challenge, especially for ApoB\u2010100, which cannot be solubilized in purified form.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>A simultaneous quantitation method for ApoA\u2010I and ApoB\u2010100 was developed using tryptic digestion without predigestion reduction and alkylation, followed by LC separation coupled with isotope dilution MS analysis. The accuracy of the method was assured by selecting structurally exposed signature peptides, optimal choice of detergent, protein:enzyme ratio, and incubation time. Peptide calibrators were value assigned by isobaric tagging isotope dilution MS amino acid analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The method reproducibility was validated in technical repeats of three serum samples, giving 2\u20133% intraday CVs (<jats:italic>N<\/jats:italic> = 5) and &lt;7% interday CVs (<jats:italic>N<\/jats:italic> = 21). The repeated analysis of interlaboratory harmonization standards showed \u22121% difference for ApoA\u2010I and \u221212% for ApoB\u2010100 relative to the assigned value. The applicability of the method was demonstrated by repeated analysis of 24 patient samples with a wide range of total cholesterol and triglyceride levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>The method is applicable for simultaneous analysis of ApoA\u2010I and ApoB\u2010100 in patient samples, and for characterization of serum pool calibrators for other analytical platforms.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201600128","type":"journal-article","created":{"date-parts":[[2017,3,10]],"date-time":"2017-03-10T15:29:16Z","timestamp":1489159756000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["High throughput quantification of apolipoproteins A\u2010I and B\u2010100 by isotope dilution MS targeting fast trypsin releasable peptides without reduction and alkylation"],"prefix":"10.1002","volume":"11","author":[{"given":"Bryan A","family":"Parks","sequence":"first","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M","family":"Schieltz","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L","family":"Andrews","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S","family":"Gardner","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon C","family":"Rees","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher A","family":"Toth","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey I","family":"Jones","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa G","family":"McWilliams","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsanna","family":"Kuklenyik","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L","family":"Pirkle","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R","family":"Barr","sequence":"additional","affiliation":[{"name":"Division of Laboratory Sciences Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2013-0358"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2016.03.010"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahj.2008.08.010"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2015.28"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000437741.48606.98"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-015-1401-8"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2006.07.007"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1093\/clinchem\/39.5.773","article-title":"International\u2010federation\u2010of\u2010clinical\u2010chemistry standardization project for measurements of apolipoprotein\u2010A\u2010I and apolipoprotein\u2010B .3. comparability of apolipoprotein\u2010A\u2010I values by use of international reference material","volume":"39","author":"Marcovina S. 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