{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T23:09:59Z","timestamp":1775257799656,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,22]],"date-time":"2018-11-22T00:00:00Z","timestamp":1542844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009700","name":"Fondazione Gigi e Pupa Ferrari","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Membranous nephropathy (MN) is the most frequent cause of nephrotic syndrome in adults and the disease course is characterized by the \u201crule of third\u201d, with one\u2010third of patients experiencing complete remission and the remaining experiencing relapses or progression of the disease. Additionally, the therapeutic approach is not standardized, leading to further heterogeneity in terms of response and outcome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental design<\/jats:title>\n                    <jats:p>\n                      In this pilot study, MALDI\u2013MSI analysis is performed on renal biopsies (\n                      <jats:italic>n<\/jats:italic>\n                      = 13) obtained from two homogeneous groups of patients, which differentially responded to the immunosuppressive treatments (Ponticelli regimen).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A signal at\n                      <jats:italic>m\/z<\/jats:italic>\n                      1303 displays the greatest discriminatory power when comparing the two groups and is observed to be of higher intensity in the glomeruli of the non\u2010responding patients. The corresponding tryptic peptide is identified as macrophage migration inhibitory factor (MIF).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and clinical relevance<\/jats:title>\n                    <jats:p>Despite much effort being made in recent years to understand the pathogenesis of MN, a biomarker able to predict the outcome of these patients following therapeutic treatment is still lacking. Here, a protein (MIF), verified by immunohistochemistry, that can differentiate between these MN patients and could be a valuable starting point for a further study focused on verifying its predictive role in therapy response is highlighted.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.201800019","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T09:25:51Z","timestamp":1540459551000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["MALDI\u2013MSI Pilot Study Highlights Glomerular Deposits of Macrophage Migration Inhibitory Factor as a Possible Indicator of Response to Therapy in Membranous Nephropathy"],"prefix":"10.1002","volume":"13","author":[{"given":"Vincenzo","family":"L'Imperio","sequence":"first","affiliation":[{"name":"Department of Medicine and Surgery, Pathology University of Milano\u2010Bicocca San Gerardo Hospital Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6530-6113","authenticated-orcid":false,"given":"Andrew","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Clinical Proteomics and Metabolomics Unit University of Milano\u2010Bicocca Vedano al Lambro Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Ajello","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Nephrology Unit University of Milano\u2010Bicocca Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella","family":"Piga","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Clinical Proteomics and Metabolomics Unit University of Milano\u2010Bicocca Vedano al Lambro Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Stella","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Clinical Proteomics and Metabolomics Unit University of Milano\u2010Bicocca Vedano al Lambro Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanna","family":"Denti","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Clinical Proteomics and Metabolomics Unit University of Milano\u2010Bicocca Vedano al Lambro Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Tettamanti","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Clinical Proteomics and Metabolomics Unit University of Milano\u2010Bicocca Vedano al Lambro Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato Alberto","family":"Sinico","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Nephrology Unit University of Milano\u2010Bicocca Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Pieruzzi","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery Nephrology Unit University of Milano\u2010Bicocca Monza Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Garozzo","sequence":"additional","affiliation":[{"name":"Department of Nephrology Santa Marta e Santa Venera Hospital Acireale Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisella","family":"Vischini","sequence":"additional","affiliation":[{"name":"Department of Nephrology Ospedale Agostino Gemelli Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Nebuloni","sequence":"additional","affiliation":[{"name":"Research Center for Renal Immunopathology University of Milan Milan Italy"},{"name":"Department of Biomedical and Clinical Sciences L. 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