{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:35:17Z","timestamp":1789644917559,"version":"4.0.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T00:00:00Z","timestamp":1531872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004012","name":"Jane ja Aatos Erkon S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004012","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>There are no blood biomarkers to detect early\u2010stage oral cavity squamous cell carcinoma (OSCC) prior to clinical signs. Most OSCC incidence is associated with significant morbidity and poor survival. The authors aimed to use mass\u2010spectrometry (MS) technology to find specific <jats:italic>N<\/jats:italic>\u2010glycopeptides potentially serving as serum biomarkers for preclinical OSCC screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>Serum samples from 14 patients treated for OSCC (stage I or stage IV) with 12 age\u2010 and sex\u2010matched controls are collected. Quantitative label\u2010free <jats:italic>N<\/jats:italic>\u2010glycoproteomics is performed, with MS\/MS analysis of the statistically significantly different <jats:italic>N<\/jats:italic>\u2010glycopeptides.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Combined with a database search using web\u2010based software (GlycopeptideID), MS\/MS provided detailed <jats:italic>N<\/jats:italic>\u2010glycopeptide information, including glycosylation site, glycan composition, and proposed structures. Thirty\u2010eight tryptic <jats:italic>N<\/jats:italic>\u2010glycopeptides are identified, having 19 unique <jats:italic>N<\/jats:italic>\u2010glycosylation sites representing 14 glycoproteins. OSCC patients, including stage I tumors, can be differentiated from healthy controls based on the expression levels of these glycoforms. <jats:italic>N<\/jats:italic>\u2010glycopeptides of IgG1, IgG4, haptoglobin, and transferrin have statistically significant different abundances between cases and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>The authors are the first to suggest specific <jats:italic>N<\/jats:italic>\u2010glycopeptides to serve as potential serum biomarkers to detect preclinical OSCC in patients. These <jats:italic>N<\/jats:italic>\u2010glycopeptides are the lead candidates for validation as future diagnostic modalities of OSCC as early as stage I.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201800061","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T07:39:46Z","timestamp":1531294786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Tongue Cancer Patients Can be Distinguished from Healthy Controls by Specific <i>N<\/i>\u2010Glycopeptides Found in Serum"],"prefix":"10.1002","volume":"12","author":[{"given":"Mayank","family":"Saraswat","sequence":"first","affiliation":[{"name":"Transplantation Laboratory University of Helsinki, Haartmaninkatu 3  P.O. Box 21 00014 Helsinki Finland"},{"name":"Hospital District of Helsinki and Uusimaa Laboratory Helsinki University Hospital  00290 Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antti","family":"M\u00e4kitie","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery University of Helsinki and Helsinki University Hospital  00130 Helsinki Finland"},{"name":"Division of Ear Nose and Throat Diseases Department of Clinical Sciences Intervention and Technology Karolinska Institutet and Karolinska Hospital  11382 Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiialotta","family":"Tohmola","sequence":"additional","affiliation":[{"name":"Transplantation Laboratory University of Helsinki, Haartmaninkatu 3  P.O. Box 21 00014 Helsinki Finland"},{"name":"Department of Biosciences University of Helsinki  P.O. 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