{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:51:31Z","timestamp":1762102291472,"version":"3.41.2"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T00:00:00Z","timestamp":1541116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81171655"],"award-info":[{"award-number":["81171655"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["1403062023"],"award-info":[{"award-number":["1403062023"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Epstein\u2010Barr virus (EBV) is a ubiquitous human gamma herpes virus that infects human epithelial cells and B lymphocytes. It would be potentially valuable to develop novel combined assays to benefit screening for large panels of samples of EBV infectious diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>A simple antigen\u2010probed biochip that is modified with S\u2010S\u2010PEG\u2010COOH and is used as a label\u2010free high\u2010throughput screening method for a combined detection of EBV capsid antigen IgM antibody, capsid antigen IgG antibody, and nuclear antigen IgG antibody.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This protein biochip has similar feasibility, sensitivity, and specificity in comparison with Liaison chemiluminescent immunoassay (CLIA). Detection limit of the EBV antibodies by the biochip is almost identical to that by CLIA\u2010L (2.91\u00a0U\u00a0mL<jats:sup>\u20131<\/jats:sup> vs 3.00\u00a0U\u00a0mL<jats:sup>\u20131<\/jats:sup> for EBNA\u20101 IgG, 8\u00a0U\u00a0mL<jats:sup>\u20131<\/jats:sup> vs10\u00a0U\u00a0mL<jats:sup>\u20131<\/jats:sup> for EBV\u2010VCA IgG, and 3.5\u00a0U\u00a0mL<jats:sup>\u20131<\/jats:sup> vs 10\u00a0U\u00a0mL<jats:sup>\u20131<\/jats:sup> for EBV\u2010VCA IgM). Tests of the three serological antibodies against EBV by the biochip are consistent with the CLIA\u2010L method in 274 clinical sera, respectively. Finally, the combined biochip is successfully utilized for diagnostic identification of EBV infection in 14 patients with infectious mononucleosis (IM) and 25 patients with systemic lupus erythematosus SLE, as well as additional 10 known real\u2010time PCR positive patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>This biochip format will enable concurrent detection of antibodies against EBV infection and confirm infection status of EBV. It will be a versatile tool for large\u2010scale epidemiological screening in view of its miniaturization and high throughput.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201800067","type":"journal-article","created":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T06:45:02Z","timestamp":1539326702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["S\u2010S\u2010PEG\u2010COOH Self\u2010Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes"],"prefix":"10.1002","volume":"13","author":[{"given":"Hui","family":"Lv","sequence":"first","affiliation":[{"name":"Department of Biology School of Life Sciences Anhui Medical University  Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Pathology Anhui Medical University  Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology Anhui Medical University  Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song\u2010Guo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology Anhui Medical University  Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory The First Affiliated Hospital Anhui Medical University  Hefei 230022 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na\u2010Li","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biology School of Life Sciences Anhui Medical University  Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology Anhui Medical University  Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Bin","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Biology School of Life Sciences Anhui Medical University  Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2056-0606","authenticated-orcid":false,"given":"Wei\u2010Dong","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Pathology Anhui Medical University  Hefei 230032 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,2]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2016.10.014"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.5.1873-1874.2006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.5230\/jgc.2016.16.1.1"},{"key":"e_1_2_8_4_1","first-page":"885","volume":"29","author":"Song C.","year":"2013","journal-title":"J. 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