{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:13:49Z","timestamp":1790172829280,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T00:00:00Z","timestamp":1536192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Systemic sclerosis (SSc) is characterized by autoimmunity, vasculopathy and fibrosis. Fibrosis is due to an activation of fibroblasts by the transforming growth factor\u2010\u00df (TGF\u2010\u00df). This study investigates the proteomic response of SSc fibroblasts to TGF\u2010\u00df.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>Skin fibroblasts from diffuse SSc patients and healthy controls (HC) are cultured with or without TGF\u2010\u00df. Two\u2010dimensional differential in\u2010gel electrophoresis and mass spectrometry (MS) combined with Ingenuity Pathway analysis (IPA) and Panther\/David software analyze proteins differentially expressed between groups. Real\u2010time cell analyzer (RTCA) assesses fibroblast proliferation and viability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two\u2010hundred\u2010and\u2010seventy\u2010nine proteins are differentially expressed between groups. Principal component analysis shows significant differences between groups. IPA shows specific process networks such as actin cytoskeleton and integrin signaling. Panther and David software show predominant biological processes such as cellular and metabolic processes. TGF\u2010\u00df enhances protein synthesis and protein pathways. IPA and RTCA suggest the involvement of epidermal growth factor receptor (EGFR) and phosphatidylinositol 3 kinase (Pi3K).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>That the proteome of fibroblasts differs between SSc patients and HC is confirmed, and it is demonstrated that fibroblasts exacerbate their proteomic phenotype upon stimulation with TGF\u2010\u00df. EGFR and Pi3K are highlighted as proteins of interest in SSc fibroblasts.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201800069","type":"journal-article","created":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T06:55:25Z","timestamp":1535093725000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Proteomic Analysis of Human Scleroderma Fibroblasts Response to Transforming Growth Factor\u2010\u00df"],"prefix":"10.1002","volume":"13","author":[{"given":"Benjamin","family":"Chaigne","sequence":"first","affiliation":[{"name":"INSERM U1016 Institut Cochin  75014 Paris France"},{"name":"CNRS UMR 8104  75014 Paris France"},{"name":"Universit\u00e9 Paris Descartes Sorbonne Paris Cit\u00e9  75014 Paris France"},{"name":"Service de M\u00e9decine Interne Centre de R\u00e9f\u00e9rence Maladies Syst\u00e9miques Autoimmunes Rares Vascularites N\u00e9crosantes Et Scl\u00e9rodermie Syst\u00e9mique H\u00f4pital Cochin Assistance Publique\u2010H\u00f4pitaux de Paris  75014 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilhem","family":"Clary","sequence":"additional","affiliation":[{"name":"INSERM U1016 Institut Cochin  75014 Paris France"},{"name":"CNRS UMR 8104  75014 Paris France"},{"name":"Proteomic core facility of Paris Descartes University (3P5)  75014 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgane","family":"Le Gall","sequence":"additional","affiliation":[{"name":"INSERM U1016 Institut Cochin  75014 Paris France"},{"name":"CNRS UMR 8104  75014 Paris France"},{"name":"Proteomic core facility of Paris Descartes University (3P5)  75014 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Dumoitier","sequence":"additional","affiliation":[{"name":"INSERM U1016 Institut Cochin  75014 Paris France"},{"name":"CNRS UMR 8104  75014 Paris France"},{"name":"Universit\u00e9 Paris Descartes Sorbonne Paris Cit\u00e9  75014 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Fernandez","sequence":"additional","affiliation":[{"name":"INSERM U1016 Institut Cochin  75014 Paris France"},{"name":"CNRS UMR 8104  75014 Paris France"},{"name":"Universit\u00e9 Paris Descartes Sorbonne Paris Cit\u00e9  75014 Paris France"},{"name":"Service de M\u00e9decine Interne Centre de R\u00e9f\u00e9rence Maladies Syst\u00e9miques Autoimmunes Rares Vascularites N\u00e9crosantes Et Scl\u00e9rodermie Syst\u00e9mique H\u00f4pital Cochin Assistance Publique\u2010H\u00f4pitaux de Paris  75014 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastien","family":"Lofek","sequence":"additional","affiliation":[{"name":"INSERM U1016 Institut Cochin  75014 Paris France"},{"name":"CNRS UMR 8104  75014 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Chafey","sequence":"additional","affiliation":[{"name":"INSERM U1016 Institut Cochin  75014 Paris France"},{"name":"CNRS UMR 8104  75014 Paris France"},{"name":"Proteomic core facility of Paris Descartes University (3P5)  75014 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pia","family":"Moinzadeh","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Cologne  50937 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Krieg","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Cologne  50937 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P.","family":"Denton","sequence":"additional","affiliation":[{"name":"Institute of Immunity and Transplantation Centre for Rheumatology and Connective Tissue Diseases Royal Free Hospital  NW3 2QG London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9838-246X","authenticated-orcid":false,"given":"Luc","family":"Mouthon","sequence":"additional","affiliation":[{"name":"INSERM U1016 Institut Cochin  75014 Paris France"},{"name":"CNRS UMR 8104  75014 Paris France"},{"name":"Universit\u00e9 Paris Descartes Sorbonne Paris Cit\u00e9  75014 Paris France"},{"name":"Service de M\u00e9decine Interne Centre de R\u00e9f\u00e9rence Maladies Syst\u00e9miques Autoimmunes Rares Vascularites N\u00e9crosantes Et Scl\u00e9rodermie Syst\u00e9mique H\u00f4pital Cochin Assistance Publique\u2010H\u00f4pitaux de Paris  75014 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,6]]},"reference":[{"key":"e_1_2_7_1_1","first-page":"e267","volume":"43","author":"Dumoitier N.","year":"2014","journal-title":"Presse M\u00e9dicale Paris Fr. 1983"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.53"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2006.066068"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0806188"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107827"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200107)44:7<1667::AID-ART291>3.0.CO;2-Y"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307380200"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116015"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2011-200955"},{"key":"e_1_2_7_10_1","author":"Dumoitier N.","year":"2016","journal-title":"Arthritis Rheumatol. Hoboken NJ"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03270"},{"key":"e_1_2_7_12_1","first-page":"1876","volume":"22","author":"Ludwicka A.","year":"1995","journal-title":"J. Rheumatol."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200111)44:11<2653::AID-ART445>3.0.CO;2-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10361"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa052955"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.137"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114017"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.bor.0000434672.77891.41"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.318"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1993.1170"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI77958"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31139"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.38098"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201500006"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2014.03.020"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200060"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.092"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/ken370"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00530.2007"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.091095"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.03.006"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-205013"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.160736"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1586\/eci.11.39"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23791"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12614"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2013.144"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.04.005"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2013.07.007"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.3109\/10799893.2013.862270"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.451336"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00522.2011"},{"key":"e_1_2_7_43_1","first-page":"1463","volume":"17","author":"Tokiyama K.","year":"1990","journal-title":"J. Rheumatol."},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100035"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2008.104299"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0847com"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11029"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.89073"}],"container-title":["PROTEOMICS \u2013 Clinical Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fprca.201800069","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.201800069","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prca.201800069","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.201800069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T14:20:12Z","timestamp":1694269212000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.201800069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,6]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1002\/prca.201800069"],"URL":"https:\/\/doi.org\/10.1002\/prca.201800069","archive":["Portico"],"relation":{},"ISSN":["1862-8346","1862-8354"],"issn-type":[{"value":"1862-8346","type":"print"},{"value":"1862-8354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,6]]},"assertion":[{"value":"2018-04-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1800069"}}