{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:12:00Z","timestamp":1790154720931,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>In matrix\u2010assisted laser desorption\/ionization\u2013mass spectrometry imaging (MALDI\u2013MSI) standardized sample preparation is important to obtain reliable results. Herein, the impact of section thickness in formalin\u2010fixed paraffin embedded (FFPE) tissue microarrays (TMA) on spectral intensities is investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>TMAs consisting of ten different tissues represented by duplicates of ten patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a0200 cores) are cut at 1, 3, and 5\u00a0\u03bcm. MSI analysis is performed and mean intensities of all evaluable cores are extracted. Measurements are merged and mean <jats:italic>m\/z<\/jats:italic> intensities are compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Visual inspection of spectral intensities between 1, 3, and 5\u00a0\u03bcm reveals generally higher intensities in thinner tissue sections. Specifically, higher intensities are observed in the vast majority of peaks (98.6%, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01) in 1\u00a0\u03bcm compared with 5\u00a0\u03bcm sections. Note that 28.4% and 2.1% of <jats:italic>m\/z<\/jats:italic> values exhibit a at least two\u2010 and threefold intensity difference (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01) in 1\u00a0\u03bcm compared to 5\u00a0\u03bcm sections, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A section thickness of 1\u00a0\u03bcm results in higher spectral intensities compared with 5\u00a0\u03bcm. The results highlight the importance of standardized protocols in light of recent efforts to identify clinically relevant biomarkers using MSI. The use of TMAs for comparative analysis seems advantageous, as section thickness displays less variability.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201800074","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T08:53:30Z","timestamp":1537088010000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["In MALDI\u2013Mass Spectrometry Imaging on Formalin\u2010Fixed Paraffin\u2010Embedded Tissue Specimen Section Thickness Significantly Influences <i>m\/z<\/i> Peak Intensity"],"prefix":"10.1002","volume":"13","author":[{"given":"R\u00e9mi","family":"Longuesp\u00e9e","sequence":"first","affiliation":[{"name":"Institute of Pathology University of Heidelberg  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Kriegsmann","sequence":"additional","affiliation":[{"name":"Department of Hematology Oncology and Rheumatology University of 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