{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T20:02:57Z","timestamp":1770753777840,"version":"3.50.0"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T00:00:00Z","timestamp":1538956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001774","name":"University of Sydney","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001171","name":"Cancer Institute NSW","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Actinic keratoses (AK) are premalignant tumors that can be clinically difficult to differentiate from skin cancer. An easy, quick, and reliable noninvasive alternative to biopsy is needed to definitively confirm the clinical diagnoses. This study evaluates Tape Stripping (TS) of stratum corneum (SC) for noninvasive biomarker analysis of AK.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Lesional and nonlesional human SC samples are obtained by application of stripping tapes on the skin of five AK patients. Following sample preparation, protein digests are analyzed by LC\u2013MS\/MS. Bioinformatics analyses are performed using Funrich, Ingenuity Pathway Analysis (IPA), and Oncomine bioinformatics and analytical tools.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the total 613 unique proteins identified, 477 overlap with proteins identified in the proteomic analysis of formalin\u2010fixed and paraffin\u2010embedded (FFPE) AK samples. Additionally, 32 proteins are significantly increased and four proteins decreased in AK samples compared to the normal skin (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). In line with proteomic analysis of FFPE samples, IPA and Funrich analysis show that differentially abundant proteins in the TS AK samples are implicated in PI3K\/AKT and EGF signaling pathways. These findings are confirmed at the transcript level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Tape stripped AK sample is suitable for biomarker analysis. The application of this technique further could revolutionize management of keratinocytic skin tumors by reducing the need for traditional invasive biopsy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201800084","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T13:25:41Z","timestamp":1537795541000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Tape Stripped Stratum Corneum Samples Prove to be Suitable for Comprehensive Proteomic Investigation of Actinic Keratosis"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0661-2601","authenticated-orcid":false,"given":"Ali","family":"Azimi","sequence":"first","affiliation":[{"name":"Centre for Translational Skin Research The University of Sydney  Westmead New South Wales 2145 Australia"},{"name":"Department of Dermatology Westmead Hospital  Westmead New South Wales 2145 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Ali","sequence":"additional","affiliation":[{"name":"Centre for Translational Skin Research The University of Sydney  Westmead New South Wales 2145 Australia"},{"name":"Department of Dermatology Westmead Hospital  Westmead New South Wales 2145 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberley L.","family":"Kaufman","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science Faculty of Science The University of Sydney  New South Wales 2006 Australia"},{"name":"Brain and Mind Centre The University of Sydney  Camperdown New South Wales 2050 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham J.","family":"Mann","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research Westmead Institute for Medical Research The University of Sydney  Westmead New South Wales 2145 Australia"},{"name":"Melanoma Institute Australia The University of Sydney  Wollstonecraft New South Wales 2065 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Fernandez\u2010Penas","sequence":"additional","affiliation":[{"name":"Centre for Translational Skin Research The University of Sydney  Westmead New South Wales 2145 Australia"},{"name":"Department of Dermatology Westmead Hospital  Westmead New South Wales 2145 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,8]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.07121.x"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)91658-3"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1991.01680060103013"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2007.03166.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24284"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1684\/ejd.2012.1874","volume":"22","author":"Du Thanh A.","year":"2012","journal-title":"Eur. 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