{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:09:21Z","timestamp":1791310161198,"version":"4.3.1"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,12,27]],"date-time":"2018-12-27T00:00:00Z","timestamp":1545868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81701522"],"award-info":[{"award-number":["81701522"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Damage to the uterosacral ligaments is an important contributor to uterine and vaginal prolapse. The aim of this study is to identify differentially expressed proteins (DEPs) in the uterosacral ligaments of women with and without pelvic organ prolapse (POP) and analyze their relationships to cellular mechanisms involved in the pathogenesis of POP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>Uterosacral ligament connective tissue from four patients with POP and four control women undergo iTRAQ analysis followed by ingenuity pathway analysis (IPA) of DEPs. DEPs are validated using Western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 1789 unique protein sequences are identified in the uterosacral ligament connective tissues. The expression levels of 88 proteins are significantly different between prolapse and control groups (\u22651.2\u2010fold, <jats:italic>p<\/jats:italic> &lt; 0.05). IPA demonstrates the association of 14 DEPs with \u201cConnective Tissue Function.\u201d Among them, fibromodulin, collagen alpha\u20101 (XIV) chain, calponin\u20101, tenascin, and galectin\u20101 appear most likely to play a role in the etiology of POP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>At least six proteins not previously associated with the pathogenesis of POP with biologic functions that suggest a plausible relationship to the disorder are identified. These results may be helpful for furthering the understanding of the pathophysiological mechanisms of POP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201800086","type":"journal-article","created":{"date-parts":[[2018,12,5]],"date-time":"2018-12-05T11:13:22Z","timestamp":1544008402000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Proteomics of Uterosacral Ligament Connective Tissue from Women with and without Pelvic Organ Prolapse"],"prefix":"10.1002","volume":"13","author":[{"given":"Xiang\u2010Juan","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Reproductive Genetics Ministry of Education Zhejiang University  Hangzhou 310058 China"},{"name":"Hangzhou Women's Hospital  Hangzhou 310008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Tao","family":"Pan","sequence":"additional","affiliation":[{"name":"Shaoxing Women and Children's Hospital  Shaoxing 312000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan\u2010Juan","family":"Chen","sequence":"additional","affiliation":[{"name":"Hangzhou Women's Hospital  Hangzhou 310008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Bin","family":"Fu","sequence":"additional","affiliation":[{"name":"Shaoxing Women and Children's Hospital  Shaoxing 312000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Fang","sequence":"additional","affiliation":[{"name":"Shaoxing Women and Children's Hospital  Shaoxing 312000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo\u2010Hua","family":"He","sequence":"additional","affiliation":[{"name":"Shaoxing Women and Children's Hospital  Shaoxing 312000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shaoxing Women and Children's Hospital  Shaoxing 312000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Gang","family":"Ding","sequence":"additional","affiliation":[{"name":"Shaoxing Women and Children's Hospital  Shaoxing 312000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yu","sequence":"additional","affiliation":[{"name":"Shaoxing Women and Children's Hospital  Shaoxing 312000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Reproductive Genetics Ministry of Education Zhejiang University  Hangzhou 310058 China"},{"name":"Department of Pathology and Pathophysiology School of Medicine Zhejiang University  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Jing","family":"Tan","sequence":"additional","affiliation":[{"name":"International Peace Maternity and Child Health Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fa\u2010Lin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Hangzhou Normal University  Hangzhou 311121 Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0793-3965","authenticated-orcid":false,"given":"Abraham N.","family":"Morse","sequence":"additional","affiliation":[{"name":"Guangzhou Women and Children's Medical Center  Guangzhou 510623 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u2010Feng","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Reproductive Genetics Ministry of Education Zhejiang University  Hangzhou 310058 China"},{"name":"International Peace Maternity and Child Health Hospital School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,27]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1002\/nau.20798","volume":"29","author":"International Urogynecological Association; International Continence Society","year":"2010","journal-title":"Neurourol. 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