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Urine proteins reflect not only renal diseases but also changes in other organs in the body. However, urine has rarely been used to reflect on inflammatory bowel disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Design<\/jats:title>\n                    <jats:p>In the present study, a trinitrobenzene sulfonic acid (TNBS)\u2010induced colitis rat model is used to mimic the human inflammatory bowel disease Crohn's disease (CD). Urine samples are analyzed by label\u2010free and TMT\u2010labeled proteomic quantitative methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>77 urinary proteins are significantly changed in the colitis rats compared with that in the controls. These proteins are further validated by parallel reaction monitoring (PRM) targeted proteomic quantitative methods. Based on the human protein tissue atlas, carbonic anhydrase 1, ribonuclease pancreatic gamma type, and neutral and basic amino acid transport protein rBAT are highly enriched in the gastrointestinal tract. Among the nine PRM\u2010validated proteins, carbonic anhydrase 1, neutrophil collagenase, and neutrophil gelatinase associated lipocalin were previously reported as IBD\u2010associated proteins (all exhibiting consistent trends with the observation herein), whereas the others are newly discovered by this study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Relevance<\/jats:title>\n                    <jats:p>The results provide valuable clues for future study of urine biomarker of inflammatory bowel disease and Crohn's disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.201800100","type":"journal-article","created":{"date-parts":[[2019,5,16]],"date-time":"2019-05-16T14:01:13Z","timestamp":1558015273000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Urine Proteome Changes in a TNBS\u2010Induced Colitis Rat Model"],"prefix":"10.1002","volume":"13","author":[{"given":"Weiwei","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Gene Engineering Drug and Biotechnology Beijing Key Laboratory Beijing Normal University  Beijing 100875 China"},{"name":"Department of Anesthesiology Qingdao Municipal Hospital  Qingdao 266073 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lujun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Gene Engineering Drug and Biotechnology Beijing Key Laboratory Beijing Normal University  Beijing 100875 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Gene Engineering Drug and Biotechnology Beijing Key Laboratory Beijing Normal University  Beijing 100875 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Gene Engineering Drug and Biotechnology Beijing Key Laboratory Beijing Normal University  Beijing 100875 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7159-2558","authenticated-orcid":false,"given":"Youhe","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Gene Engineering Drug and Biotechnology Beijing Key Laboratory Beijing Normal University  Beijing 100875 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,28]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)32448-0"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(16)30077-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e318247c32f"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2007.02.015"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520600554527"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crohns.2012.09.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i1.21"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjw168"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201400120"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2017.01.059"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2015.04.013"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2017.1322904"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2012.01.001"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i12.3231"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euprot.2014.02.010"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-013-4574-1"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-014-4661-y"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.010975"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2015.08.005"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2017.03.028"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.2015.1094380"},{"key":"e_1_2_7_22_1","volume":"1","author":"Gao Y.","year":"2014","journal-title":"MOJPB"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09314"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-12-42"},{"key":"e_1_2_7_25_1","volume":"11","author":"Wu J.","year":"2017","journal-title":"Proteomics Clin. 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