{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T19:25:51Z","timestamp":1783625151664,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T00:00:00Z","timestamp":1583193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","award":["Strategic funds from Karolinska Institutet"],"award-info":[{"award-number":["Strategic funds from Karolinska Institutet"]}],"id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>To decipher the underlying immunological mechanisms in predisposition to oral microbial dysbiosis in severe congenital neutropenia (SCN) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>Ten SCN patients (5\u201323 years old) and 12 healthy controls (5\u201322 years old) are periodontally examined and provided saliva, subgingival plaque, and gingival crevicular fluid (GCF) samples. The SCN patients received oral hygiene therapy and are re\u2010evaluated after 6 months. Antimicrobial peptides HPN1\u20103 and LL\u201037 are assessed in saliva by ELISA. Concentration of 30 cytokines is measured in saliva and GCF by human 30\u2010plex panel, while bacterial profiles of saliva and subgingival plaque are assessed using 16S rDNA amplicon sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There is no significant difference in salivary HPN1\u20103 and LL\u201037 concentration between the SCN patients and controls. At baseline, clinical, immunological, and microbiological parameters of the patients are indicative of oral ecological dysbiosis. The SCN patients have significantly higher bleeding on probing (BOP)%, GCF volume, and cytokine levels, high bacterial load with low bacterial diversity in saliva. The associations between the microbiome and immunological parameters in the SCN patients differ from those in the healthy individuals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>SCN patients have a dysregulated immune response toward commensal oral microbiota, which could be responsible for the observed clinical and microbiological signs of dysbiosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201900058","type":"journal-article","created":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T01:03:52Z","timestamp":1580951032000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Dysbiosis of the Oral Ecosystem in Severe Congenital Neutropenia Patients"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1432-6194","authenticated-orcid":false,"given":"Egija","family":"Zaura","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry Academic Centre for Dentistry Amsterdam (ACTA) Vrije Universiteit Amsterdam and University of Amsterdam 1600NP Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd W.","family":"Brandt","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry Academic Centre for Dentistry Amsterdam (ACTA) Vrije Universiteit Amsterdam and University of Amsterdam 1600NP Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Buijs","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry Academic Centre for Dentistry Amsterdam (ACTA) Vrije Universiteit Amsterdam and University of Amsterdam 1600NP Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fclnur","family":"Emingil","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Dentistry Ege University \u0130zmir 35100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merve","family":"Erg\u00fcz","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Dentistry Ege University \u0130zmir 35100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deniz Yilmaz","family":"Karapinar","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology School of Medicine Ege University \u0130zmir 35100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bur\u00e7","family":"Pekpinarli","sequence":"additional","affiliation":[{"name":"Department of Pediatrics School of Dentistry Ege University \u0130zmir 35100 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1183-5607","authenticated-orcid":false,"given":"Kai","family":"Bao","sequence":"additional","affiliation":[{"name":"Division of Oral Diseases Department of Dental Medicine Karolinska Institutet Huddinge 14104 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8164-0653","authenticated-orcid":false,"given":"Georgios N.","family":"Belibasakis","sequence":"additional","affiliation":[{"name":"Division of Oral Diseases Department of Dental Medicine Karolinska Institutet Huddinge 14104 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagihan","family":"Bostanci","sequence":"additional","affiliation":[{"name":"Division of Oral Diseases Department of Dental Medicine Karolinska Institutet Huddinge 14104 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,3]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05008.x"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.32"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1956.tb06875.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2001.tb02801.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050191"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-09-045427"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.9.1499"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513507956"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000964"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-011-9572-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11201-3"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"e27923","DOI":"10.1002\/pbc.27923","volume":"66","author":"Y. 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