{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T09:32:33Z","timestamp":1778923953192,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T00:00:00Z","timestamp":1574812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Diffuse large B\u2010cell lymphoma (DLBCL), the most common non\u2010Hodgkin lymphoma, is a heterogeneous lymphoma with different clinical manifestations and molecular alterations, and several markers are currently being measured routinely for its diagnosis, subtyping, or prognostication by immunohistochemistry (IHC). Here, the utility of a reverse\u2010phase\u2010protein\u2010array (RPPA) as a novel supportive tool to measure multiple biomarkers for DLBCL diagnosis is validated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>The expression of seven markers (CD5, CD10, BCL2, BCL6, MUM1, Ki\u201067, and C\u2010MYC) is analyzed by RPPA and IHC using 37 DLBCL tissues, and the correlation between the two methods is determined. To normalize tumor content ratio in the tissues, the raw RPPA values of each marker are adjusted by that of CD20 or PAX\u20105.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The CD20\u2010adjusted data for CD5, MUM1, BCL2, Ki\u201067, and C\u2010MYC has better correlation with IHC results than PAX\u20105\u2010adjusted data. Receiver operating characteristic (ROC) analysis reveals that CD5, MUM1, BCL2, and C\u2010MYC exhibit a better sensitivity and specificity &gt;0.750. Furthermore, the CD20\u2010adjusted C\u2010MYC value strongly correlates with that of IHC, and has a particularly high specificity (0.882).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>Although further investigation using a large number of DLBCL specimens needs to be conducted, these results suggest that RPPA could be applicable as a supportive tool for determining lymphoma prognosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201900091","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T13:49:08Z","timestamp":1573652948000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Utility of a Reverse Phase Protein Array to Evaluate Multiple Biomarkers in Diffuse Large B\u2010Cell Lymphoma"],"prefix":"10.1002","volume":"14","author":[{"given":"Masaki","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Pathology Kanagawa Cancer Center  Yokohama 241\u20108515 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Muroi","sequence":"additional","affiliation":[{"name":"Division of Cancer Cell Research Kanagawa Cancer Center Research Institute  Yokohama 241\u20108515 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanori","family":"Nojima","sequence":"additional","affiliation":[{"name":"Center for Translational Research The Institute of Medical Science Hospital University of Tokyo  Tokyo 108\u20108639 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayumi","family":"Numata","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Medical Oncology Kanagawa Cancer Center  Yokohama 241\u20108515 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotaka","family":"Takasaki","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Medical Oncology Kanagawa Cancer Center  Yokohama 241\u20108515 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rika","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Medical Oncology Kanagawa Cancer Center  Yokohama 241\u20108515 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Yokose","sequence":"additional","affiliation":[{"name":"Department of Pathology Kanagawa Cancer Center  Yokohama 241\u20108515 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Miyagi","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology and Genetics Kanagawa Cancer Center Research Institute  Yokohama 241\u20108515 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4539-888X","authenticated-orcid":false,"given":"Naohiko","family":"Koshikawa","sequence":"additional","affiliation":[{"name":"Division of Cancer Cell Research Kanagawa Cancer Center Research Institute  Yokohama 241\u20108515 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,27]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.11.3909"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1545"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800396"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000501"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.4.1145"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.3.815"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq627"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.12810"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.41.4342"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.41.0985"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033813"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-06-435842"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-460063"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12763"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104068"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095020"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-1907-z"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12942"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/1.3.305"},{"key":"e_1_2_7_20_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow S. 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