{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:20:07Z","timestamp":1791570007625,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T00:00:00Z","timestamp":1596585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Early diagnosis is crucial to increase the chances of conservation treatment for patients with steroid\u2010induced osteonecrosis of the femoral head (SIONFH). This study aimed to identify serum peptides as potential biomarkers to diagnose SIONFH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>The serum proteome of 32 SIONFH patients and 24 healthy controls are analyzed using magnetic bead\u2010based weak cation exchange (MB\u2010WCX) and matrix\u2010assisted laser desorption ionization\u2010time of flight mass spectrometry (MALDI\u2010TOF\u2010MS). Next, candidate biomarkers are identified using liquid chromatography\u2010electrospray ionization\u2010tandem mass spectrometry (LC\u2010ESI\u2010MS\/MS). Candidate biomarkers are then validated using ELISA and western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>39 peaks are identified and the expression fold changes of seven peaks in the two groups are greater than 1.5. Three peaks (m\/z: 1077.84\u00a0Da; m\/z: 1061.78\u00a0Da; m\/z: 1099.56\u00a0Da) tend to be upregulated, while four peaks (m\/z: 3973.92\u00a0Da; m\/z: 7766.53\u00a0Da; m\/z: 3957.31\u00a0Da; m\/z: 4212.02\u00a0Da) tend to be down\u2010regulated in SIONFH patients. The peak for a 1077.84\u00a0Da peptide is identified as Isoform 1 of the Fibrinogen alpha chain precursor (FGA). ELISAs and western blot analyses reveal that the expression of FGA is significantly higher in SIONFH patients than healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Clinical Relevance<\/jats:title><jats:p>FGA is overexpressed in SIONFH patients, and thus, is a novel potential biomarker for SIONFH.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201900099","type":"journal-article","created":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T07:41:30Z","timestamp":1594971690000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Isoform 1 of Fibrinogen Alpha Chain Precursor is a Potential Biomarker for Steroid\u2010Induced Osteonecrosis of the Femoral Head"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2201-0210","authenticated-orcid":false,"given":"Liying","family":"Liu","sequence":"first","affiliation":[{"name":"The Center Laboratory for Biomedical Research Xi'an Jiaotong University Health Science Center  Xi'an Shaanxi Province 710061 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jidong","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi Province 710004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi Province 710004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Huang","sequence":"additional","affiliation":[{"name":"Core Research Laboratory The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi Province 710004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Yang","sequence":"additional","affiliation":[{"name":"The Center Laboratory for Biomedical Research Xi'an Jiaotong University Health Science Center  Xi'an Shaanxi Province 710061 China"},{"name":"Department of Cell Biology and Genetics, School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center, Shaanxi  Xi'an Shaanxi Province 710061 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shugang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi Province 710004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi Province 710004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital of Xi'an Jiaotong University  Xi'an Shaanxi Province 710004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,5]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2008.10.001"},{"key":"e_1_2_8_1_2","first-page":"125","author":"Spencer J. 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