{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:37:59Z","timestamp":1788579479331,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["BCRT"],"award-info":[{"award-number":["BCRT"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Discrimination between ulcerative colitis (UC) and Crohn's disease (CD) by histologic features alone can be challenging and often leads to inaccurate initial diagnoses in inflammatory bowel disease (IBD) patients. This is mostly due to an overlap of clinical and histologic features. However, exact diagnosis is not only important for patient treatment but it also has a socioeconomic impact. It is therefore important to develop and improve diagnostic tools complementing traditional histomorphological approaches.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>In this retrospective proof\u2010of\u2010concept study, the utilization of MALDI imaging is explored in combination with multi\u2010variate data analysis methods to classify formalin\u2010fixed, paraffin\u2010embedded (FFPE) colon biopsies from UC (87 biopsies, 14 patients), CD (71 biopsies, 14 patients), and normal colonic (21 biopsies, 14 patients) tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The proposed method results in an overall balanced accuracy of 85.7% on patient and of 80.4% on sample level, thus demonstrating that the assessment of IBD from FFPE tissue specimens via MALDI imaging is feasible.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>The results emphasize the high potential of this method to distinguish IBD subtypes in FFPE tissue sections, which is a prerequisite for further investigations in retrospective multicenter studies, as well as for a future implementation into clinical routine.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.201900131","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T11:23:50Z","timestamp":1595330630000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Classification of Inflammatory Bowel Disease from Formalin\u2010Fixed, Paraffin\u2010Embedded Tissue Biopsies via Imaging Mass Spectrometry"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7984-7607","authenticated-orcid":false,"given":"Oliver","family":"Klein","sequence":"first","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Augustenburger Platz 1 13353 Berlin Germany"},{"name":"Berlin\u2010Brandenburg Center for Regenerative Therapies Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Augustenburger Platz 1 13353 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"Fogt","sequence":"additional","affiliation":[{"name":"Penn Presbyterian Medical Center Hospital of the University of Pennsylvania  51N 39th Street Philadelphia PA 19104 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Hollerbach","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology AKH Celle  Siemensplatz 4 29223 Celle Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grit","family":"Nebrich","sequence":"additional","affiliation":[{"name":"Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Corporate member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health  Augustenburger Platz 1 13353 Berlin Germany"},{"name":"Berlin\u2010Brandenburg Center for Regenerative Therapies Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Augustenburger Platz 1 13353 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Boskamp","sequence":"additional","affiliation":[{"name":"Center for Industrial Mathematics University of Bremen  Bibliothekstr. 5 28359 Bremen Germany"},{"name":"SCiLS Bruker Daltonik GmbH  Fahrenheitstr. 4 28359 Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Wellmann","sequence":"additional","affiliation":[{"name":"Institute of Pathology  Wittinger Strasse 14 29223 Celle Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,4]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crohns.2013.01.010"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i1.21"},{"key":"e_1_2_8_3_1","first-page":"826","volume":"7","author":"Tremaine W. 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