{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T03:22:47Z","timestamp":1792207367199,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T00:00:00Z","timestamp":1592870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Although the pathophysiological response of cardiac tissue to pro\u2010hypertrophic stimulus is well characterized, a comprehensive characterization of the molecular events underlying the pathological hypertrophy in cardiomyocytes during the early compensated cardiac hypertrophy is currently lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>A quantitative label\u2010free proteomic analysis of cardiomyocytes isolated was conducted from mice treated subcutaneously with isoproterenol (ISO) during 7 days in comparison with cardiomyocytes from control animals (CT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Canonical pathway analysis of dysregulated proteins indicated that ISO\u2010hypertrophy drives the activation of actin cytoskeleton and integrin\u2010linked kinase (ILK) signaling, and inhibition of the sirtuin signaling. Alteration in cardiac contractile function and calcium signaling are predicted as downstream effects of ISO\u2010hypertrophy probably due to the upregulation of key elements such as myosin\u20107 (MYH7). Confocal microscopy corroborated that indeed ISO\u2010treatment led to increased abundance of MYH7. Potential early markers for cardiac hypertrophy as APBB1, GOLGA4, HOOK1, KATNA1, KIFBP, MAN2B2, and SLC16A1 are also reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>The data consist in a complete molecular mapping of ISO\u2010induced compensated cardiac hypertrophy model at cardiomyocyte level. Marker candidates reported may assist early diagnosis of cardiac hypertrophy and ultimately heart failure.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202000017","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T17:53:40Z","timestamp":1591552420000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Cardiomyocyte Proteome Remodeling due to Isoproterenol\u2010Induced Cardiac Hypertrophy during the Compensated Phase"],"prefix":"10.1002","volume":"14","author":[{"given":"Ricardo Cambraia","family":"Parreira","sequence":"first","affiliation":[{"name":"Departamento de Fisiologia e Biof\u00edsica, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais 31270\u2010901 Brazil"},{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Minas 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