{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:40:16Z","timestamp":1761766816576,"version":"3.41.2"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T00:00:00Z","timestamp":1603065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Ureteropelvic junction obstruction (UPJO) is the most frequent cause of congenital hydronephrosis in child. To better investigate the molecular mechanisms of this pathological process, the stenotic ureter proteome of UPJO in infants is compared with their own normal pre\u2010stenotic segments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>Data independent acquisition\u2010based proteomics are performed to compare proteome between pre\u2010stenotic and stenotic ureter from nine UPJO infants. Gene ontology analysis, hierarchical cluster analysis, and network interaction are performed to characterize biological functions of significantly altered proteins. Selected significantly altered proteins are validated by western blot on another three UPJO infants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>15 proteins are up\u2010regulated and 33 proteins are down\u2010regulated during stenotic pathology. Significantly altered proteins are involved in decreased extracellular matrix and cytoskeleton organization, increased regulation of oxidative activity, and altered inflammatory associated exocytosis. Significant expression of biglycan, fibulin\u20101, myosin\u201010, cytochrome b5 are validated providing possible mechanism in UPJO which could be associated impaired smooth muscle cell, epithelial integrity, and increased oxidative stress.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical\u00a0Relevance<\/jats:title><jats:p>This study provides molecular evidence of dysregulated extracellular matrix organization, impaired smooth muscle cell, and oxidative stress during UPJO pathology, indicating that biglycan, fibulin\u20101, myosin\u201010, cytochrome b5 might reflect the pathology of UPJO.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202000030","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T08:06:23Z","timestamp":1600934783000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4083-4571","authenticated-orcid":false,"given":"Yueyan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhoutong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qimin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maosheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guofeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4722-416X","authenticated-orcid":false,"given":"Hongquan","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoliang","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Urology Xin hua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  1665 Kongjiang Road Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,19]]},"reference":[{"key":"e_1_2_9_1_1","first-page":"585","volume":"18","author":"Chevalier R. 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