{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:40:28Z","timestamp":1785476428141,"version":"3.56.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Histopathological evaluation presents conflicting reports regarding aortic abnormalities. The authors aim to present proof\u2010of\u2010concept study to explore the feasibility of matrix\u2010assisted laser desorption\/ionization imaging mass spectrometry (MALDI\u2010IMS) in combination with histopathology for characterizing alterations in the aneurysmal ascending formalin\u2010fixed paraffin\u2010embedded (FFPE) aorta tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>The authors assess FFPE specimens from patients with a dilated aorta and bicuspid aortic valve (BAV), those with a standard tricuspid aortic valve (TAV), and those with Marfan syndrome (MFS) via histopathology and grade the conditions for elastic fiber fragmentation (EFF) and MALDI\u2010IMS. The proteins using liquid chromatographic\u2013mass spectrometry are identified and the results are confirmed by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There is significant difference in terms of EFF between MFS and BAV, and TAV and BAV. Characteristic peptide signatures and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> values in the EFF facilitate the characterization among the aortic specimens of BAV, MFS, and TAV. The <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> values from the aortic alpha smooth muscle actin and myosin heavy chains significantly increase in BAV compared with MFS and TAV. These findings are confirmed by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results represent a strategy that uses MALDI\u2010IMS in combination with histopathology as promising approaches to characterize spatial alteration in the structure of the aneurysmal ascending aorta.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202000047","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T16:48:34Z","timestamp":1607014114000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of the Aortopathy in the Ascending Aorta: The Novelty of Using Matrix\u2010Assisted Laser Desorption\/Ionization Imaging"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4365-8956","authenticated-orcid":false,"given":"Salah A.","family":"Mohamed","sequence":"first","affiliation":[{"name":"Department of Cardiac and Thoracic Vascular Surgery UKSH\u2010Campus Luebeck  Luebeck 23538 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliane T.","family":"Taube","sequence":"additional","affiliation":[{"name":"Charit\u00e9\u2010Universitaetsmedizin Institute for Pathology  Berlin 10117 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Thiele","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Digital Medicine MEVIS  Luebeck 23538 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Noack","sequence":"additional","affiliation":[{"name":"Institute of Pathology Martin\u2010Luther Hospital  Berlin 14193 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grit","family":"Nebrich","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health Center for Regenerative Therapies &amp; Berlin\u2010Brandenburg Center for Regenerative Therapies (BCRT) Campus Virchow Klinikum (CVK) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin 13353 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal","family":"Mohamady","sequence":"additional","affiliation":[{"name":"Asklepios\u2010Klinikum Harburg  Hamburg 21075 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten","family":"Hanke","sequence":"additional","affiliation":[{"name":"Asklepios\u2010Klinikum Harburg  Hamburg 21075 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7984-7607","authenticated-orcid":false,"given":"Oliver","family":"Klein","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health Center for Regenerative Therapies &amp; Berlin\u2010Brandenburg Center for Regenerative Therapies (BCRT) Campus Virchow Klinikum (CVK) Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin 13353 Germany"},{"name":"German Center for Cardiovascular Research (DZHK) Partner site Berlin  Berlin 13353 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.14.5.400"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/4.suppl_1.1799"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.31872"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2006.10.065"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2006.10.074"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2008.06.027"},{"key":"e_1_2_9_7_1","first-page":"2","volume-title":"Cardiac Surgery","author":"Mohamed S. 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