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Peptides from iris were analysed using liquid chromatography\u2010tandem mass spectrometry on an Orbitrap Q Exactive Plus mass spectrometer. Verification of proteins of interest was conducted using selected reaction monitoring on a triple quadrupole mass spectrometer. The main outcome was proteins with a log<jats:sub>2<\/jats:sub> two\u2010fold difference in expression in iris between PACG and controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were 3,446 non\u2010redundant proteins identified in human iris, of which 416 proteins were upregulated and 251 proteins were downregulated in PACG compared with controls. Thirty\u2010two upregulated proteins were either components of the extracellular matrix (ECM) (fibrillar collagens, EMILIN\u20102, fibrinogen, fibronectin, matrilin\u20102), matricellular proteins (thrombospondin\u20101), proteins involved in cell\u2010matrix interactions (integrins, laminin, histidine\u2010rich glycoprotein, paxillin), or protease inhibitors known to modulate ECM turnover (\u03b1\u20102 macroglobulin, tissue factor pathway inhibitor 2, papilin). Two giant proteins, titin and obscurin, were up\u2010 and down\u2010regulated, respectively, in the iris in PACG compared with controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>This proteomic study shows that ECM composition and homeostasis are altered in the iris in PACG.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202000094","type":"journal-article","created":{"date-parts":[[2021,7,9]],"date-time":"2021-07-09T09:43:02Z","timestamp":1625823782000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Primary angle closure glaucoma is characterized by altered extracellular matrix homeostasis in the iris"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8350-2908","authenticated-orcid":false,"given":"Richard D.","family":"Semba","sequence":"first","affiliation":[{"name":"Wilmer Eye Institute Johns Hopkins University School of 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