{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:17:07Z","timestamp":1791404227221,"version":"4.3.3"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T00:00:00Z","timestamp":1632441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT 156111"],"award-info":[{"award-number":["PJT 156111"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["CGS\u2010D"],"award-info":[{"award-number":["CGS\u2010D"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Comparative genomics and phenotypic assays have shown that antibiotic resistance profiles differ among clinical isolates of <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and that genotype\u2010phenotype associations are difficult to establish for resistance phenotypes based on these comparisons alone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>Here, we used label\u2010free quantitative proteomics to compare two isolates of the Liverpool Epidemic Strain (LES) of <jats:italic>P. aeruginosa<\/jats:italic>, LESlike1 and LESB58, and the common laboratory strain <jats:italic>P. aeruginosa<\/jats:italic> PAO1 to more accurately predict functional differences between strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results show that the proteomes of the LES isolates are more similar to each other than to PAO1; however, a number of differences were observed in the abundance of proteins involved in quorum sensing, virulence, and antibiotic resistance, including in the comparison of LESlike1 and LESB58. Additionally, the proteomic data revealed a higher abundance of proteins involved in polymyxin and aminoglycoside resistance in LESlike1. Minimum inhibitory concentration assays showed that LESlike1 had up to 128\u2010fold higher resistance to antibiotics from these classes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings provide an example of the ability of proteomic data to complement genotypic and phenotypic studies to understand resistance in clinical isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Relevance<\/jats:title><jats:p><jats:italic>P. aeruginosa<\/jats:italic> is a predominant pathogen in chronic lung infections in individuals with cystic fibrosis (CF). LES isolates are capable of transferring between CF patients and have been associated with increased hospital visits and antibiotic treatments.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202100062","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T17:26:55Z","timestamp":1631467615000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Label\u2010free quantitative proteomics identifies unique proteomes of clinical isolates of the Liverpool Epidemic Strain of <i>Pseudomonas aeruginosa<\/i> and laboratory strain PAO1"],"prefix":"10.1002","volume":"15","author":[{"given":"Mara C.","family":"Goodyear","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Garnier","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan R.","family":"Krieger","sequence":"additional","affiliation":[{"name":"Bioinformatics Solutions, Inc  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Geddes\u2010McAlister","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cezar M.","family":"Khursigara","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,24]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Cystic Fibrosis Canada(2018). 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