{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T22:24:37Z","timestamp":1769293477575,"version":"3.49.0"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T00:00:00Z","timestamp":1667174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Peritoneal carcinomatosis (PC), metastasized from colorectal cancer (CRC), remains a highly lethal disease. Outcomes of PC is significantly influenced by the amount of intra\u2010abdominal tumor burden and therefore diagnostic tests that facilitate earlier diagnosis could improve PC treatment and patient outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>Using mass\u2010spectrometry\u2010based proteomics, we characterized the protein features of circulating exosomes in the context of CRC PC, CRC with liver metastasis, and primary CRC limited to the colon. We profiled exosomes isolated from patient plasma to identify exosome\u2010associated protein cargoes released by these cancer types.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Analysis of the resulting data identified metastasis\u2010specific exosome protein signatures. Bioinformatic analyses confirmed enrichment of proteins annotated to vesicle\u2010associated processes and intracellular compartments, as well as representation of cancer hallmark functions and processes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Clinical Relevance<\/jats:title><jats:p>This research yielded distinct protein profiles for the CRC patient groups and suggests the utility of plasma exosome proteomic analysis for a better understanding of PC development and metastasis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202100085","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T08:44:52Z","timestamp":1665477892000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Exosomal proteins as a source of biomarkers in colon cancer\u2010derived peritoneal carcinomatosis \u2013 A pilot study"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7627-6984","authenticated-orcid":false,"given":"Paul A.","family":"Vallejos","sequence":"first","affiliation":[{"name":"Department of Basic Sciences Center for Health Disparities &amp; Molecular Medicine Division of Biochemistry Loma Linda University Medical Center  Loma Linda California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan N.","family":"Fuller","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Center for Health Disparities &amp; Molecular Medicine Division of Biochemistry Loma Linda University Medical Center  Loma Linda California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janviere","family":"Kabagwira","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Center for Health Disparities &amp; Molecular Medicine Division of Biochemistry Loma Linda University Medical Center  Loma Linda California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei Li","family":"Kwong","sequence":"additional","affiliation":[{"name":"Department of General Surgery National Institutes of Health  Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amber","family":"Gonda","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Center for Health Disparities &amp; Molecular Medicine Division of Biochemistry Loma Linda University Medical Center  Loma Linda California USA"},{"name":"Department of Surgery Division of Surgical Oncology University of California at Irvine  Orange California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James R. W.","family":"McMullen","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Center for Health Disparities &amp; Molecular Medicine Division of Biochemistry Loma Linda University Medical Center  Loma Linda California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3235-4386","authenticated-orcid":false,"given":"Natasha","family":"Le","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Center for Health Disparities &amp; Molecular Medicine Division of Biochemistry Loma Linda University Medical Center  Loma Linda California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew J.","family":"Selleck","sequence":"additional","affiliation":[{"name":"Department of Surgery Mountain View Hospital  Las Vegas Nevada USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lance D.","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology Wake Forest School of Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher C.","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Center for Health Disparities &amp; Molecular Medicine Division of Biochemistry Loma Linda University Medical Center  Loma Linda California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maheswari","family":"Senthil","sequence":"additional","affiliation":[{"name":"Department of Surgery Division of Surgical Oncology University of California at Irvine  Orange California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan R.","family":"Wall","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Center for Health Disparities &amp; Molecular Medicine Division of Biochemistry Loma Linda University Medical Center  Loma Linda California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,10,31]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(20000115)88:2<358::AID-CNCR16>3.0.CO;2-O"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104613\u20101247\u20105_4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470\u20102045(16)30500\u20109"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.20049"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.10527"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470\u20102045(20)30599\u20104"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00384\u2010014\u20101907\u2010x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434\u2010008\u20109966\u20102"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16480"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2019.3616"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434\u2010018\u20106561\u2010z"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.14670\/hh\u201030.43"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010010\u20100534\u20104"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046737"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/07357907.2015.1081921"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102407\u201014\u2010176"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2008.04.033"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/1177271917715236"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12307\u2010018\u20100209\u20101"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22126258"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19021568"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201300282"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201016\u20100360"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2016.1156535"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11051\u2010021\u201005169\u20106"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092921"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16103"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S291956"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1088\/2057-1976\/aad6d8"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt703"},{"key":"e_1_2_8_33_1","unstructured":"R Core Team. (2021).A language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria."},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00218.2019"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00521"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9080931"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900210"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.202000068"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300534"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clcc.2015.12.002"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.553297"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434\u2010015\u20104778\u20107"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25030619"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11102323"},{"issue":"1","key":"e_1_2_8_45_1","first-page":"12","article-title":"Role of proteins of the macroglobulin family in regulation of tumor growth]","volume":"37","author":"Zorin N. A.","year":"2006","journal-title":"Ontogenez"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1159\/000024517"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541\u20107786.MCR\u201019\u20101033"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0215909"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.13021"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2013.04.065"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pan.2020.06.010"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13730\u2010021\u201000602\u20100"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11390"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8111343"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23238"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.612622"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.03.009"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2015.18"},{"issue":"5","key":"e_1_2_8_59_1","first-page":"4161","article-title":"Expression of apolipoprotein A1 in colonic adenocarcinoma","volume":"23","author":"Tachibana M.","year":"2003","journal-title":"Anticancer Research"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.13.3.443"}],"container-title":["PROTEOMICS \u2013 Clinical Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.202100085","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prca.202100085","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.202100085","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T17:57:42Z","timestamp":1692640662000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.202100085"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,31]]},"references-count":59,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/prca.202100085"],"URL":"https:\/\/doi.org\/10.1002\/prca.202100085","archive":["Portico"],"relation":{},"ISSN":["1862-8346","1862-8354"],"issn-type":[{"value":"1862-8346","type":"print"},{"value":"1862-8354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,31]]},"assertion":[{"value":"2021-09-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2100085"}}