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The difference in their physiological functions or the different dynamic ranges of these proteins in plasma makes it difficult to detect them simultaneously and to use several of these proteins as a tool in clinical practice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>A novel multiplex assay has been designed and optimized to carry out a high\u2010throughput and simultaneous screening of APRs, allowing the detection of each of them at the same time and in their corresponding dynamic range.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using Sars\u2010CoV\u20102 infection as a model, it has been possible to profile different patterns of acute phase proteins that vary significantly between healthy and infected patients. In addition, severity profiles (acute respiratory distress syndrome and sepsis) have been established.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>Differential profiles in acute phase proteins can serve as a diagnostic and prognostic tool, among patient stratification. The design of this new platform for their simultaneous detection paves the way for them to be more extensive use in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202100100","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T08:21:06Z","timestamp":1664353266000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Systematic evaluation of plasma signaling cascades by functional proteomics approaches: SARS\u2010CoV\u20102 infection as model"],"prefix":"10.1002","volume":"16","author":[{"given":"Pablo","family":"Juanes\u2010Velasco","sequence":"first","affiliation":[{"name":"Department of Medicine and Cytometry General Service\u2010Nucleus, CIBERONC Cancer Research Centre (IBMCC\/CSIC\/USAL\/IBSAL)  Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina 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