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This is the first study describing the serum autoantibody profile of PAs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>We performed serological autoantibody profiling of four healthy controls, four Acromegaly, three Cushing's and three NFPAs patient samples to obtain their autoantibody profiles, which were used for studying expression, interaction and altered biological pathways. Further, significant autoantibodies of PAs were compared with data available for glioma, meningioma and AAgAtlas for their specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Autoantibody profile of PAs led to the identification of differentially expressed significant proteins such as AKNAD1 (AT\u2010Hook Transcription Factor [AKNA] Domain Containing 1), NINJ1 (Nerve injury\u2010induced protein 1), L3HYPDH (Trans\u20103\u2010hydroxy\u2010L\u2010proline dehydratase), RHOG (Rho\u2010related GTP\u2010binding protein) and PTP4A1 (Protein Tyrosine Phosphatase Type IVA 1) in Acromegaly. Protein ABR (Active breakpoint cluster region\u2010related protein), ST6GALNAC6 (ST6 <jats:italic>N<\/jats:italic>\u2010acetylgalactosaminide alpha\u20102, 6\u2010sialyltransferase 6), NOL3 (Nucleolar protein 3), ANXA8 (Annexin A8) and POLR2H (RNA polymerase II, I and III subunit H) showed an antigenic response in Cushing's patient's serum samples. Protein dipeptidyl peptidase 3 (DPP3) and reticulon\u20104 (RTN4) exhibited a very high antigenic response in NFPA patients. These proteins hold promise as potential autoantibody biomarkers in PAs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202100111","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T16:34:52Z","timestamp":1659976492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of autoantibody signatures in pituitary adenoma patients using human proteome arrays"],"prefix":"10.1002","volume":"16","author":[{"given":"Arghya","family":"Banerjee","sequence":"first","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Powai Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arka","family":"Ray","sequence":"additional","affiliation":[{"name":"Centre for Research in Nanotechnology &amp; Science (CRNTS) Indian Institute of Technology Bombay  Powai Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhilash","family":"Barpanda","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Powai Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Dash","sequence":"additional","affiliation":[{"name":"Miranda House University of Delhi University Enclave  New Delhi Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishika","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Engineering University Institute of Engineering and Technology Panjab University  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehar Un","family":"Nissa","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Powai Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Molecular Sciences\/High\u2010Throughput Biology Center Johns Hopkins University School of Medicine  Baltimore, Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhidha","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery at King Edward Memorial Hospital and Seth G. 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