{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T14:13:23Z","timestamp":1789827203059,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31870823"],"award-info":[{"award-number":["31870823"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["M21003"],"award-info":[{"award-number":["M21003"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To develop a robust microarray platform to detect thousands of serological autoantibodies (AAbs) simultaneously in different diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Experimental Design<\/jats:title>\n                    <jats:p>An AAbMap microarray was prepared by printing a total of 4032 purified His\u2010tagged human proteins and peptide probes on a chemically\u2010modified slide. The sensitivity, dynamic range, and the inter\u2010 and intra\u2010array reproducibility of the AAb microarray were then systematically tested and optimized. Finally, the large\u2010scale profiling of AAbs in the serum of patients with different human diseases using the AAbMap microarray was demonstrated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The dynamic range of antibody (Ab) detection was 2 to 3 orders of magnitude with the lowest limit of detection (LOD) of 68 pg\/mL. The intra\u2010array (\n                      <jats:italic>r<\/jats:italic>\n                      ) correlation of duplicate spots was 1.00, whereas the inter\u2010array correlations between different arrays and batches were 0.99 and 0.97 to 0.98, respectively. Notably, 132, 266, 171, and 84 AAbs were detected in pooled serum from healthy controls (HCs) or patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), or lung cancer (LC), respectively. These AAbs included antibodies that target well\u2010known disease biomarkers, such as anti\u2010cyclic citrullinated peptide, anti\u2010ribonucleoprotein, and anti\u2010nucleosome.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions and Clinical Relevance<\/jats:title>\n                    <jats:p>We developed a microarray platform to measure thousands of serological AAbs simultaneously with high sensitivity and reproducibility. The array can help study autoimmunity and complement genomics, proteomics, and metabolomics data for systematic investigations of human diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/prca.202100132","type":"journal-article","created":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T12:53:16Z","timestamp":1661431996000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Development of a protein microarray for profiling circulating autoantibodies in human diseases"],"prefix":"10.1002","volume":"16","author":[{"given":"Jing","family":"Ren","sequence":"first","affiliation":[{"name":"School of Basic Medicine Sciences Anhui Medical University  Hefei Anhui PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongye","family":"Wang","sequence":"additional","affiliation":[{"name":"National Center for Protein Sciences Beijing (PHOENIX Center), State Key Laboratory of Proteomics, Beijing Proteome Research Center Beijing Institute of Lifeomics  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chundi","family":"Wei","sequence":"additional","affiliation":[{"name":"National Center for Protein Sciences Beijing (PHOENIX Center), State Key Laboratory of Proteomics, Beijing Proteome Research Center Beijing Institute of Lifeomics  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Sciences Anhui Medical University  Hefei Anhui PR China"},{"name":"National Center for Protein Sciences Beijing (PHOENIX Center), State Key Laboratory of Proteomics, Beijing Proteome Research Center Beijing Institute of Lifeomics  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Yu","sequence":"additional","affiliation":[{"name":"National Center for Protein Sciences Beijing (PHOENIX Center), State Key Laboratory of Proteomics, Beijing Proteome Research Center Beijing Institute of Lifeomics  Beijing PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.119.044408"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2018.56"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14388"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/db20\u20100937"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398\u2010021\u201001596\u20100"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw946"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2017.61"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2020.101388"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis\u20102020\u2010218460"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021933"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.45816"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.044"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2016.08.143"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.051250"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.1c00630"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr100686b"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051931"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kez634"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M116.061002"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.645632"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbt.2015.09.002"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","unstructured":"Nevers Y. 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