{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T13:31:26Z","timestamp":1772717486446,"version":"3.50.1"},"reference-count":71,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:00:00Z","timestamp":1696291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["ICMR\/SRF\/2019\u20104232"],"award-info":[{"award-number":["ICMR\/SRF\/2019\u20104232"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Hypoxic Ischemic Encephalopathy (HIE) is one of the principal causes of neonatal mortality and long\u2010term morbidity worldwide. The neonatal signs of mild cerebral injury are subtle, making an early precise diagnosis difficult. Delayed detection, poor prognosis, and lack of specific biomarkers for the disease are increasing mortality rates. In this study, we intended to identify specific biomarkers using comparative proteomic analysis to predict the severity of perinatal asphyxia so that its outcome can also be prevented.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>A case\u2010control study was conducted on 38 neonates, and urine samples were collected within 24 and 72\u00a0h of life. A tandem mass spectrometry\u2010based quantitative proteomics approach, followed by validation via sandwich ELISA, was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The LC\u2010MS\/MS\u2010based proteomics analysis resulted in the identification of 1201 proteins in urine, with 229, 244, and 426 being differentially expressed in HIE\u20101, HIE\u20102, and HIE\u20103, respectively. Axon guidance, Diseases of programmed cell death, and Detoxification of reactive oxygen species pathways were significantly enriched in mild HIE versus severe HIE. Among the differentially expressed proteins in various stages of HIE, we chose to validate four proteins \u2013 APP, AGT, FABP1, and FN1 \u2013 via sandwich ELISA. Individual and cumulative ROC curves were plotted. AGT and FABP1 together showed high sensitivity, specificity, and accuracy as potential biomarkers for early diagnosis of HIE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Establishing putative urinary biomarkers will facilitate clinicians to more accurately screen neonates for brain injury and monitor the disease progression. Prompt treatment of neonates may reduce mortality and neurodevelopmental impairment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202200054","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T08:41:19Z","timestamp":1696408879000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A comparative proteomic analysis for non\u2010invasive early prediction of hypoxic\u2010ischemic injury in asphyxiated neonates"],"prefix":"10.1002","volume":"18","author":[{"given":"Sumrati","family":"Gurtoo","sequence":"first","affiliation":[{"name":"Center for Systems Biology and Molecular Medicine Yenepoya Research Centre Yenepoya (Deemed to be University)  Mangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gayathree","family":"Karthikkeyan","sequence":"additional","affiliation":[{"name":"Center for Systems Biology and Molecular Medicine Yenepoya Research Centre Yenepoya (Deemed to be University)  Mangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santosh Kumar","family":"Behera","sequence":"additional","affiliation":[{"name":"Center for Systems Biology and Molecular Medicine Yenepoya Research Centre Yenepoya (Deemed to be University)  Mangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chinmaya Narayana","family":"Kotimoole","sequence":"additional","affiliation":[{"name":"Center for Systems Biology and Molecular Medicine Yenepoya Research Centre Yenepoya (Deemed to be University)  Mangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd. 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