{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T05:40:12Z","timestamp":1772257212953,"version":"3.50.1"},"reference-count":69,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Colorectal cancer (CRC) has been reported as the second leading cause of cancer death worldwide. The 5\u2010year annual survival is around 50%, mainly due to late diagnosis, striking necessity for early detection. This study aims to identify autoantibody in patients\u2019 sera for early screening of cancer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>The study used a high\u2010density human proteome array with approximately 17,000 recombinant proteins. Screening of sera from healthy individuals, CRC from Indian origin, and CRC from middle\u2010east Asia origin were performed. Bio\u2010statistical analysis was performed to identify significant autoantibodies altered. Pathway analysis was performed to explore the underlying mechanism of the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The comprehensive proteomic analysis revealed dysregulation of 15 panels of proteins including CORO7, KCNAB1, WRAP53, NDUFS6, KRT30, and COLGALT2. Further biological pathway analysis for the top dysregulated autoantigenic proteins revealed perturbation in important biological pathways such as ECM degradation and cytoskeletal remodeling etc.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Clinical Relevance<\/jats:title><jats:p>The generation of an autoimmune response against cancer\u2010linked pathways could be linked to the screening of the disease. The process of immune surveillance can be detected at an early stage of cancer. Moreover, AAbs can be easily extracted from blood serum through the least invasive test for disease screening.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202200062","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T10:47:57Z","timestamp":1669027677000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A protein microarray\u2010based serum proteomic investigation reveals distinct autoantibody signature in colorectal cancer"],"prefix":"10.1002","volume":"17","author":[{"given":"Abhilash","family":"Barpanda","sequence":"first","affiliation":[{"name":"Centre for Research in Nanotechnology &amp; Science (CRNTS) Indian Institute of Technology Bombay  Mumbai India"},{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaitanya","family":"Tuckley","sequence":"additional","affiliation":[{"name":"Centre for Research in Nanotechnology &amp; Science (CRNTS) Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arka","family":"Ray","sequence":"additional","affiliation":[{"name":"Centre for Research in Nanotechnology &amp; Science (CRNTS) Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arghya","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siddhartha P.","family":"Duttagupta","sequence":"additional","affiliation":[{"name":"Centre for Research in Nanotechnology &amp; Science (CRNTS) Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chetan","family":"Kantharia","sequence":"additional","affiliation":[{"name":"Department of surgical gastroenterology at King Edward Memorial Hospital and Seth G. S. Medical College  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5159-6834","authenticated-orcid":false,"given":"Sanjeeva","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Centre for Research in Nanotechnology &amp; Science (CRNTS) Indian Institute of Technology Bombay  Mumbai India"},{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(19)32319\u20100"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.01284"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(19)30345-0"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.5114\/wo.2013.39995"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.0106"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13438"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.03.030"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.24418"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1602237"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7057-5_21"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500404102"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1562-1_14"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16997"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201600104"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.200780123"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.903033"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.645632"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.202100132"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/wsbm.118"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.O111.016253"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20107\u2010539"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3AN01577G"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/microarrays4020098"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471140864.ps2701s72"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00019-09"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.543947"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/microarrays1020064"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1562-1_18"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13895"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920\u2010019\u20100632\u20102"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110873"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.21037\/tcr.2019.07.25"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.658922"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw946"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.202100111"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201011\u20100199"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2017.10.022"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.01081"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.42507"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201500096"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13112718"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010017\u20101559\u20102"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10010009"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/846985"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13167\u2010019\u201000185\u2010y"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2012.04.017"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2021.101051"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.013297"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604680200"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.03.035"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.11.6272"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.102.033357"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000521"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.01.028"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165357"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.02085\u201007"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1133427"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.25542"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00380"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010pathol\u2010012414\u2010040438"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22115822"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature19754"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010021\u201003984\u20102"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5652"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.20892\/j.issn.2095-3941.2016.0086"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1600200"}],"container-title":["PROTEOMICS \u2013 Clinical Applications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.202200062","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/prca.202200062","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/prca.202200062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T17:57:54Z","timestamp":1692640674000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/prca.202200062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,29]]},"references-count":69,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/prca.202200062"],"URL":"https:\/\/doi.org\/10.1002\/prca.202200062","archive":["Portico"],"relation":{},"ISSN":["1862-8346","1862-8354"],"issn-type":[{"value":"1862-8346","type":"print"},{"value":"1862-8354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,29]]},"assertion":[{"value":"2022-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2200062"}}