{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T04:21:53Z","timestamp":1783138913225,"version":"3.54.6"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p><jats:italic>Helcococcus kunzii<\/jats:italic> is a skin commensal, Gram\u2010positive bacterium, mostly isolated from infected chronic wounds. This opportunistic pathogen is usually co\u2010isolated with <jats:italic>Staphylococcus aureus<\/jats:italic>. The present dataset explores the production and secretion of <jats:italic>H. kunzii<\/jats:italic> bacterial virulence interacting proteins in a growth medium mimicking chronic wounds in exponential and stationary growth phases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>The <jats:italic>H. kunzii<\/jats:italic> cellular proteome and exoproteome were assessed by analyzing three biological replicates per condition tested. Samples were analyzed using a Q\u2010Exactive HF mass spectrometer. Comparative and functional analyses were performed to profile the identified protein set.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:italic>H. kunzii<\/jats:italic>\u2019s cellular proteome encompassed 969 proteins, among which 64 and 53 were specifically identified in the exponential and stationary phase of growth, respectively. Its exoproteome comprised 58 proteins, among which 16 and 14 were characteristic of each growth stage. Metabolic differences between the two phases of growth are discussed. Besides, the production of previously shortlisted and novel putative <jats:italic>H. kunzii<\/jats:italic> targets involved in modulating the virulence of <jats:italic>S. aureus<\/jats:italic> is investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and Clinical Relevance<\/jats:title><jats:p>This work, pioneering the study of <jats:italic>H<\/jats:italic>. <jats:italic>kunzii<\/jats:italic> physiology in a chronic wound\u2010like environment, should assist future research on this opportunistic pathogen and the search for innovative approaches for wound management.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202200069","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T04:39:38Z","timestamp":1681706378000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Proteomic insights into <i>Helcococcus kunzii<\/i> in a diabetic foot ulcer\u2010like environment"],"prefix":"10.1002","volume":"17","author":[{"given":"Benjamin A. 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N.","family":"Durand","sequence":"first","affiliation":[{"name":"Bacterial Virulence and Chronic Infections INSERM U1047 University of Montpellier Department of Microbiology and Hospital Hygiene, University Hospital N\u00eemes  N\u00eemes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Dunyach\u2010Remy","sequence":"additional","affiliation":[{"name":"Bacterial Virulence and Chronic Infections INSERM U1047 University of Montpellier Department of Microbiology and Hospital Hygiene, University Hospital N\u00eemes  N\u00eemes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumayma","family":"El Kaddouri","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, CEA, INRAE D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS), SPI  Bagnols\u2010sur\u2010C\u00e8ze 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SPI  Bagnols\u2010sur\u2010C\u00e8ze France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5560-1717","authenticated-orcid":false,"given":"Lucia","family":"Grenga","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, CEA, INRAE D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9 (DMTS), SPI  Bagnols\u2010sur\u2010C\u00e8ze 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