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Clinical samples collected at diagnosis were divided into discovery (<jats:italic>n<\/jats:italic>\u00a0=\u00a030) and validation (<jats:italic>n<\/jats:italic>\u00a0=\u00a098) sets. We also analysed a set of preclinical sera (<jats:italic>n<\/jats:italic>\u00a0=\u00a030) collected prior to HGSOC diagnosis in the UK Collaborative Trial of Ovarian Cancer Screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A 7\u2010lectin LeMBA\u2010MS\/MS discovery screen shortlisted 59 candidate proteins and three lectins. Validation analysis using 3\u2010lectin LeMBA\u2010multiple reaction monitoring (MRM) confirmed elevated A1AT, AACT, CO9, HPT and ITIH3 and reduced A2MG, ALS, IBP3 and PON1 glycoforms in HGSOC. The best performing multimarker signature had 87.7% area under the receiver operating curve, 90.7% specificity and 70.4% sensitivity for distinguishing HGSOC from benign and healthy groups. 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