{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:03:01Z","timestamp":1767772981096,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,8,13]],"date-time":"2023-08-13T00:00:00Z","timestamp":1691884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics Clinical Apps"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Urine proteome is a valuable reservoir of biomarkers for disease diagnosis and monitoring. Following formation as the plasma filtrate in the kidney, urine is progressively modified by the active reabsorption and secretion of the urinary tract. However, little is known about how the urine proteome changes as it passes along the urinary tract.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental design<\/jats:title><jats:p>To investigate this, we compared the proteome composition of the renal pelvis urine (RPU) and individually self\u2010voided bladder urine (BU) collected from seven unilateral urinary tract obstruction male patients by LC\u2010MS\/MS screening. To our knowledge, this is the first proteomic comparison of RPU and BU samples from the same individual.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, RPU and BU proteomes did not exhibit proteins that were exclusively present in all samples of one urine type while in none of the other type. Nonetheless, BU had more overrepresented proteins that were observed at a higher frequency than RPU. Label\u2010free quantitative analyses revealed BU\u2013RPU differential proteins that are enriched in exosomes and extracellular proteins. However, the differences were not significant after corrections for multiple testing. Interestingly, we observed a significant increase of collagen peptides with hydroxyproline modifications in the BU samples, suggesting differences in protein modifications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and clinical relevance<\/jats:title><jats:p>Our study revealed no substantial differences at the protein level between the BU and RPU samples. Future investigations with expanded cohorts would provide more insights about the urothelial\u2013urinary interactions.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202300004","type":"journal-article","created":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T01:06:16Z","timestamp":1691975176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Quantitative comparison of the renal pelvic urine and bladder urine to examine modifications of the urine proteome by the lower urinary tract"],"prefix":"10.1002","volume":"18","author":[{"given":"Yilin","family":"Pan","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, and Tung Biomedical Sciences Center City University of Hong Kong  Kowloon Hong Kong SAR China"},{"name":"Key Laboratory of Biochip Technology Biotech and Health Centre City University of Hong Kong Shenzhen Research Institute  Shenzhen China"},{"name":"Department of Precision Diagnostic and Therapeutic Technology City University of Hong Kong Futian Research Institute  Shenzhen Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine Yim\u2010Ping","family":"Wong","sequence":"additional","affiliation":[{"name":"S.H. Ho Urology Centre, Department of Surgery The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiying","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, and Tung Biomedical Sciences Center City University of Hong Kong  Kowloon Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan Tsz\u2010Hei","family":"Tse","sequence":"additional","affiliation":[{"name":"S.H. Ho Urology Centre, Department of Surgery The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol Ka\u2010Lo","family":"Cheng","sequence":"additional","affiliation":[{"name":"S.H. Ho Urology Centre, Department of Surgery The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miaomiao","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, and Tung Biomedical Sciences Center City University of Hong Kong  Kowloon Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter Ka\u2010Fung","family":"Chiu","sequence":"additional","affiliation":[{"name":"S.H. Ho Urology Centre, Department of Surgery The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy Yuen\u2010Chun","family":"Teoh","sequence":"additional","affiliation":[{"name":"S.H. 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