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Although cystoscopy is the standard protocol for diagnosing BLCA clinically, this procedure is invasive and expensive. Several urine\u2010based markers for BLCA have been identified and investigated, but none has shown sufficient sensitivity and specificity. These observations underscore the importance of discovering novel BLCA biomarkers and developing a noninvasive method for detection of BLCA. Exploring the cancer secretome is a good starting point for the development of noninvasive biomarkers for cancer diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>In this study, we established a comprehensive secretome dataset of five representative BLCA cell lines, BFTC905, TSGH8301, 5637, MGH\u2010U1, and MGH\u2010U4, by liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS). Expression of BLCA\u2010specific secreted proteins at the transcription level was evaluated using the Oncomine cancer microarray database.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expressions of four candidates\u2014COMT, EWSR1, FUSIP1, and TNPO2\u2014were further validated in clinical human specimens. Immunohistochemical analyses confirmed that transportin\u20102 was highly expressed in tumor cells relative to adjacent noncancerous cells in clinical tissue specimens from BLCA patients, and was significantly elevated in BLCA urine compared with that in urine samples from aged\u2010matched hernia patients (controls).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Collectively, our findings suggest TNPO2 as a potential noninvasive tumor\u2010stage or grade discriminator for BLCA management.<\/jats:p><\/jats:sec>","DOI":"10.1002\/prca.202300033","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T05:39:38Z","timestamp":1704865178000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Integration of the cancer cell secretome and transcriptome reveals potential noninvasive diagnostic markers for bladder cancer"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0063-4965","authenticated-orcid":false,"given":"Yi\u2010Ting","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences College of Medicine Chang Gung University  Taoyuan Taiwan"},{"name":"Graduate Institute of Biomedical Sciences College of Medicine Chang Gung University  Taoyuan Taiwan"},{"name":"Kidney Research Center Department of Nephrology LinKou Chang Gung Memorial Hospital  Taoyuan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Ju","family":"Tu","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Sciences College of Medicine Chang Gung University  Taoyuan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zong\u2010Han","family":"Ye","sequence":"additional","affiliation":[{"name":"Graduate Institute of Biomedical Sciences College of Medicine Chang Gung University  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